Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
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Journal ArticleDOI
GSDMD membrane pore formation constitutes the mechanism of pyroptotic cell death
Lorenzo Sborgi,Sebastian Rühl,Estefania Mulvihill,Joka Pipercevic,Rosalie Heilig,Henning Stahlberg,Christopher J. Farady,Daniel J. Müller,Petr Broz,Sebastian Hiller +9 more
TL;DR: Overall, these data demonstrate that GSDMD is the direct and final executor of pyroptotic cell death.
Journal ArticleDOI
Imaging modes of atomic force microscopy for application in molecular and cell biology
Yves F. Dufrêne,Toshio Ando,Ricardo Garcia,David Alsteens,David Martinez-Martin,Andreas Engel,Christoph Gerber,Daniel J. Müller +7 more
TL;DR: The basic principles, advantages and limitations of the most common AFM bioimaging modes are reviewed, including the popular contact and dynamic modes, as well as recently developed modes such as multiparametric, molecular recognition, multifrequency and high-speed imaging.
Journal ArticleDOI
Tau protein liquid–liquid phase separation can initiate tau aggregation
Susanne Wegmann,Bahareh Eftekharzadeh,Katharina Tepper,Katarzyna Marta Zoltowska,Rachel E. Bennett,Simon Dujardin,Pawel R. Laskowski,Danny MacKenzie,Tarun V. Kamath,Caitlin Commins,Charles R. Vanderburg,Allyson D. Roe,Zhanyun Fan,Amandine Molliex,Amayra Hernández-Vega,Daniel J. Müller,Anthony A. Hyman,Eckhard Mandelkow,Eckhard Mandelkow,J. Paul Taylor,J. Paul Taylor,Bradley T. Hyman +21 more
TL;DR: It is demonstrated that phosphorylated or mutant aggregation prone recombinant tau undergoes LLPS, as does high molecular weight soluble phospho‐tau isolated from human Alzheimer brain, and it is suggested that LLPS represents a biophysical process with a role in multiple different neurodegenerative diseases.
Journal ArticleDOI
Atomic force microscopy-based mechanobiology
Michael Krieg,Gotthold Fläschner,David Alsteens,Benjamin M. Gaub,Wouter H. Roos,Gijs J.L. Wuite,Hermann E. Gaub,Christoph Gerber,Yves F. Dufrêne,Daniel J. Müller +9 more
TL;DR: The potential of combining AFM with complementary techniques, including optical microscopy and spectroscopy of mechanosensitive fluorescent constructs, super-resolution microscopy, the patch clamp technique and the use of microstructured and fluidic devices to characterize the 3D distribution of mechanical responses within biological systems and to track their morphology and functional state as discussed by the authors.
Journal ArticleDOI
Bacterial adhesion at the single-cell level
TL;DR: Understanding of the mechanisms governing bacterial adhesion at the single-cell level is summarized, including the physical forces experienced by a cell before reaching the surface, the first contact with a surface and the transition from reversible to permanent adhesion.
References
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Journal ArticleDOI
Spatially resolved force spectroscopy of biological surfaces using the atomic force microscope
William F. Heinz,Jan H. Hoh +1 more
TL;DR: The atomic force microscope can be used to produce spatially resolved maps of the surface or material properties of a sample, which will provide fundamental insights into biological structure and will become an important tool for characterizing technologically important biological systems.
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Atomic-Scale Distribution of Water Molecules at the Mica-Water Interface Visualized by Three-Dimensional Scanning Force Microscopy
TL;DR: This 3D-SFM image obtained at a mica-water interface visualizes 3D distributions of adsorbed water molecules above the center of hexagonal cavities and the laterally distributed hydration layer.
Journal ArticleDOI
Single-molecule recognition imaging microscopy
C. Stroh,Hongda Wang,R. Bash,Brian Ashcroft,Jeremy Nelson,Hermann J. Gruber,D. Lohr,Stuart Lindsay,Peter Hinterdorfer +8 more
TL;DR: The results presented here demonstrate that the highly specific antibody-antigen interaction can be used to generate single-molecule maps of specific types of molecules in a compositionally complex sample while simultaneously carrying out high-resolution topographic imaging.
Journal ArticleDOI
High-speed atomic force microscopy coming of age
TL;DR: This review describes a historical overview of technical development towards HS-AFM, summarizes elementary devices and techniques used in the current HS- AFM, and then highlights recent imaging studies.
Journal ArticleDOI
Nanoscale mapping and functional analysis of individual adhesins on living bacteria
Vincent Dupres,Franco D. Menozzi,Camille Locht,Brian H. Clare,Nicholas L. Abbott,Stéphane Cuenot,Coralie Bompard,Dominique Raze,Yves F. Dufrêne +8 more
TL;DR: A method, based on atomic force microscopy with tips bearing biologically active molecules, for measuring the specific binding forces of individual adhesins and for mapping their distribution on the surface of living bacteria.