Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
More filters
Journal ArticleDOI
Nanomechanical 3D Depth Profiling of Collagen Fibrils in Native Tendon.
TL;DR: Using atomic force microscopy (AFM) to determine the three-dimensional depth profiles of the local nanomechanical properties of collagen fibrils and their embedding interfibrillar matrix in native (unfixed), hydrated Achilles tendon of sheep and chickens sheds new light on the role of interfibrillsar bonds and the mechanical properties of the interfibrilla matrix in the biomechanics of tendon.
Book ChapterDOI
Nanostructured biomaterials with antimicrobial activity for tissue engineering
TL;DR: This chapter describes initially the mechanisms of microbial adhesion, growth, and biofilm formation since such knowledge is essential for developing antimicrobial strategies.
Journal ArticleDOI
Nanomechanical properties of composite protein networks of erythroid membranes at lipid surfaces.
Mario Encinar,Santiago Casado,Alicia Calzado-Martín,Paolo Natale,Álvaro San Paulo,Montserrat Calleja,Marisela Vélez,Marisela Vélez,Francisco Monroy,Iván López-Montero +9 more
TL;DR: A detailed nanomechanical study of individual protein microfilaments forming the protein networks of eGUVs when spread on supporting surfaces and the observed protein softening and reassembling could be relevant for understanding the mechanisms of cytoskeleton reorganization found in pathological erythrocytes or ery Throatcytes that are affected by biological agents.
Journal ArticleDOI
Indentation with atomic force microscope, Saccharomyces cerevisiae cell gains elasticity under ethanol stress
TL;DR: Atomic force microscope-based strategy was performed to determine Young's modulus of S. cerevisiae to directly clarify ethanol stress-associated changes and roles of S., which indicated that membrane composition varies under ethanol stress induced the S. Cerevisiae membrane fluidity and elasticity changes.
Journal ArticleDOI
Multiparametric characterization of heterogeneous soft materials using contact point detection-based atomic force microscopy
TL;DR: In this paper, a new algorithm was proposed to detect contact points (CPs) from each FD curve and to determine properties such as stiffness, adhesion, and deformation.
References
More filters
Journal ArticleDOI
Models for the specific adhesion of cells to cells
TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
Journal ArticleDOI
Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
Journal ArticleDOI
Single-molecule mechanics of mussel adhesion
TL;DR: A single-molecule study of the substrate and oxidation-dependent adhesive properties of dopa is reported, in which dopa exploits a remarkable combination of high strength and chemical multifunctionality to accomplish adhesion to substrates of widely varying composition.
Journal ArticleDOI
Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.