Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
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Book ChapterDOI
Probing Single Virus Binding Sites on Living Mammalian Cells Using AFM.
TL;DR: This chapter presents how FD-based AFM permits to investigate virus binding to living mammalian cells and quantify the kinetic and thermodynamic parameters that describe the free-energy landscape of the single virus-receptor-mediated binding.
Journal ArticleDOI
Advanced Microtexture Evaluation of Dextran Biofilms Obtained from Low Cost Substrate Loaded with Maytenus rigida Extract
Mário R. P. da Silva,Robert Saraiva Matos,Erveton P. Pinto,Samuel Bruno dos Santos,Michael Douglas Santos Monteiro,Henrique Duarte da Fonseca Filho,Luiz Eduardo Almeida +6 more
TL;DR: In this paper, the influence of the addition of the extract on the biofilms microtexture using stereometric and fractal parameters was evaluated, and the results indicated that this biofilm have promising 3D micromorphology for regenerative medicine application.
Journal ArticleDOI
Deciphering Single-Bacterium Adhesion Behavior Modulated by Extracellular Electron Transfer.
Shuomeng Zhang,Lei Wang,Liang Wu,Zhongjian Li,Bin Yang,Yang Hou,Lecheng Lei,Shaoan Cheng,Qinggang He +8 more
TL;DR: In this article, a nonlinear model between bacterial adhesion force and electric field intensity is established, which provides a theoretical foundation for precise tuning of bacterial strength by the surface potential and the direction and flux of electron flow.
Journal ArticleDOI
The effect of growth temperature on the nanoscale biochemical surface properties of Yersinia pestis
TL;DR: Using nanoscale imaging coupled with functional mapping, it is illustrated that changes in the surfaces of the bacterium from a morphological and biochemical standpoint can be analyzed simultaneously using atomic force microscopy and demonstrated that adaptations to different environments can be effectively captured using this technique.
Journal ArticleDOI
Imaging and quantifying analysis the binding behavior of PD-L1 at molecular resolution by atomic force microscopy
Juan Qin,Juan Qin,Miaomiao Zhang,Miaomiao Zhang,Yanxue Guan,Yanxue Guan,Xinyue Guo,Xinyue Guo,Zongjia Li,Zongjia Li,Christian Rankl,Jilin Tang,Jilin Tang +12 more
TL;DR: In this paper, the authors investigated the interaction of PD-L1 with its antibody and PD-1 using atomic force microscopy-based single molecule force spectroscopy for the first time.
References
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Journal ArticleDOI
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Journal ArticleDOI
Force measurements with the atomic force microscope: Technique, interpretation and applications
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Journal ArticleDOI
Single-molecule mechanics of mussel adhesion
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Journal ArticleDOI
Nanomechanical analysis of cells from cancer patients
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Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.