Journal ArticleDOI
Multiparametric imaging of biological systems by force-distance curve-based AFM.
TLDR
The principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions are discussed.Abstract:
A current challenge in the life sciences is to understand how biological systems change their structural, biophysical and chemical properties to adjust functionality. Addressing this issue has been severely hampered by the lack of methods capable of imaging biosystems at high resolution while simultaneously mapping their multiple properties. Recent developments in force-distance (FD) curve–based atomic force microscopy (AFM) now enable researchers to combine (sub)molecular imaging with quantitative mapping of physical, chemical and biological interactions. Here we discuss the principles and applications of advanced FD-based AFM tools for the quantitative multiparametric characterization of complex cellular and biomolecular systems under physiological conditions.read more
Citations
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Journal ArticleDOI
Evaluation of nanostructure and microstructure of bone regenerated by BMP-2-porous scaffolds.
Carlos del Rosario,María Rodríguez-Évora,Ricardo Reyes,Alejandro González-Orive,Alberto Hernández-Creus,Kevin M. Shakesheff,Lisa J. White,Araceli Delgado,Carmen Évora +8 more
TL;DR: To evaluate the nano and microstructure of the regenerated bone a multi-technique analysis was used, including stereo microscope, X-Ray; AFM, micro-CT, and histological analyses, which indicated that the main chemical elements in the new bone were oxygen, calcium, and phosphorus in a ratio similar to that of native bone.
Journal ArticleDOI
Carbon Ion-Irradiated Hepatoma Cells Exhibit Coupling Interplay between Apoptotic Signaling and Morphological and Mechanical Remodeling
TL;DR: A theoretical model of apoptosis revealed that mechanical changes in cells induce the characteristic cellular budding of apoptotic bodies, which contributes to the understanding of cancer-killing mechanisms in the context of carbon ion radiotherapy.
Journal ArticleDOI
Nanostructure and mechanical properties of the osteocyte lacunar-canalicular network-associated bone matrix revealed by quantitative nanomechanical mapping
Shuai Zhang,Fiona Linnea Bach-Gansmo,Dan Xia,Flemming Besenbacher,Henrik Birkedal,Mingdong Dong +5 more
TL;DR: In this paper, the authors used quantitative nanomechanical mapping, backscattered electron imaging, and nanoindentation to characterize the lacunar-canalicular network (LCN)-associated bone matrix.
Journal ArticleDOI
Studying RNAP-promoter interactions using atomic force microscopy.
TL;DR: The basic setup of AFM is illustrated, followed by an introduction of the applications of the techniques, including high-speed AFM (HS-AFM) imaging, to investigate RNAP-DNA interactions with a special focus on promoter-search processes and open promoter formations.
BookDOI
The Biophysics of Cell Membranes
TL;DR: This chapter discusses recent evidences supporting the existence of submicrometric lipid domains and documented mechanisms involved in their control, and provides key recent advances supporting the role of lipid C. Léonard et al. domains in cell (re)shaping.
References
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TL;DR: The force required to separate two cells is shown to be greater than the expected electrical forces between cells, and of the same order of magnitude as the forces required to pull gangliosides and perhaps some integral membrane proteins out of the cell membrane.
Journal ArticleDOI
Force measurements with the atomic force microscope: Technique, interpretation and applications
TL;DR: The atomic force microscope (AFM) is not only used to image the topography of solid surfaces at high resolution but also to measure force-versus-distance curves as discussed by the authors, which provide valuable information on local material properties such as elasticity, hardness, Hamaker constant, adhesion and surface charge densities.
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Single-molecule mechanics of mussel adhesion
TL;DR: A single-molecule study of the substrate and oxidation-dependent adhesive properties of dopa is reported, in which dopa exploits a remarkable combination of high strength and chemical multifunctionality to accomplish adhesion to substrates of widely varying composition.
Journal ArticleDOI
Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
Journal ArticleDOI
Calculation of thermal noise in atomic force microscopy
Hans-Jürgen Butt,Manfred Jaschke +1 more
TL;DR: In this paper, the authors calculated the thermal noise of a cantilever with a free end by considering all possible vibration modes of the cantilevers and showed that if the end is supported by a hard surface, no thermal fluctuations of the deflection are possible.