Potential functional applications of extracellular vesicles: a report by the NIH Common Fund Extracellular RNA Communication Consortium
Peter J. Quesenberry,Jason M. Aliotta,Giovanni Camussi,Asim B. Abdel-Mageed,Sicheng Wen,Laura R. Goldberg,Huang-Ge Zhang,Ciro Tetta,Jeffrey L. Franklin,Robert J. Coffey,Kirsty Danielson,Vinita Subramanya,Ionita Ghiran,Saumya Das,Clark C. Chen,Kae M. Pusic,Aya D. Pusic,Devasis Chatterjee,Richard P. Kraig,Leonora Balaj,Mark S. Dooner +20 more
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TLDR
The NIH Extracellular RNA Communication Program's initiative on clinical utility of extracellular RNAs and therapeutic agents and developing scalable technologies is reviewed here, and the tremendous clinical potential of EVs underlies many of these projects.Abstract:
The NIH Extracellular RNA Communication Program's initiative on clinical utility of extracellular RNAs and therapeutic agents and developing scalable technologies is reviewed here. Background information and details of the projects are presented. The work has focused on modulation of target cell fate by extracellular vesicles (EVs) and RNA. Work on plant-derived vesicles is of intense interest, and non-mammalian sources of vesicles may represent a very promising source for different therapeutic approaches. Retro-viral-like particles are intriguing. Clearly, EVs share pathways with the assembly machinery of several other viruses, including human endogenous retrovirals (HERVs), and this convergence may explain the observation of viral-like particles containing viral proteins and nucleic acid in EVs. Dramatic effect on regeneration of damaged bone marrow, renal, pulmonary and cardiovascular tissue is demonstrated and discussed. These studies show restoration of injured cell function and the importance of heterogeneity of different vesicle populations. The potential for neural regeneration is explored, and the capacity to promote and reverse neoplasia by EV exposure is described. The tremendous clinical potential of EVs underlies many of these projects, and the importance of regulatory issues and the necessity of general manufacturing production (GMP) studies for eventual clinical trials are emphasized. Clinical trials are already being pursued and should expand dramatically in the near future.read more
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RNA delivery by extracellular vesicles in mammalian cells and its applications.
Killian P. O’Brien,Koen Breyne,Stefano Ughetto,Stefano Ughetto,Louise C. Laurent,Xandra O. Breakefield +5 more
TL;DR: This Review focuses on the current state of knowledge pertaining to packaging, transport and function of RNAs in extracellular vesicles and outlines the progress made thus far towards their clinical applications.
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Plant derived edible nanoparticles as a new therapeutic approach against diseases
TL;DR: In this review, recent significant developments pertaining to plant derived edible nanoparticles are discussed and insight is provided into the use of plants as a bio-renewable, sustainable, diversified platform for the production of therapeutic nanoparticles.
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RNA Biomarkers: Frontier of Precision Medicine for Cancer.
TL;DR: The latest studies on various types of RNA biomarkers, especially extracellular RNAs, in cancer diagnosis and prognosis are summarized, and several well-known RNA biomarker of clinical utility are illustrated.
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Identification and characterization of EGF receptor in individual exosomes by fluorescence-activated vesicle sorting.
James N. Higginbotham,Qin Zhang,Dennis K. Jeppesen,Andrew M. Scott,H. Charles Manning,Josiah Ochieng,Jeffrey L. Franklin,Robert J. Coffey +7 more
TL;DR: It is proposed that FAVS may be a useful tool to monitor EGFR and AREG in circulating exosomes from individuals with colorectal cancer and possibly other solid tumours.
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Arrowtail RNA for Ligand Display on Ginger Exosome-like Nanovesicles to Systemic Deliver siRNA for Cancer Suppression
TL;DR: The application of arrowtail RNA nanoparticles for displaying ligands on ginger derived exosome-like nanovesicles (GDENs) for siRNA delivery and tumor inhibition through IV administration reveals the potential of GDENs as an economic delivery system for si RNA.
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