Journal ArticleDOI
Quantification of total genomic DNA and selected repetitive sequences reveals concurrent changes in different DNA families in indica and japonica rice
TLDR
The increase or decrease in the copy number of several repetitive sequences examined here may indicate the existence of a directed change in genome size in rice.Abstract:
This paper describes a fluorescence in situ hybridization (FISH) analysis of three different repetitive sequence families, which were mapped to mitotic metaphase chromosomes and extended DNA fibers (EDFs) of the two subspecies of rice (OrYza sativa), indica and japonica (2n = 2x = 24). The repeat families studied were (1) the tandem repeat sequence A (TrsA), a functionally non-significant repeat; (2) the [TTTA-GGG]n telomere sequence, a non-transcribed, tandemly repeated but functionally significant repeat; and (3) the 5S ribosomal RNA (5S rDNA). FISH of the TrsA repeat to metaphase chromosomes of indica and japonica cultivars revealed clear signals at the distal ends of twelve and four chromosomes, respectively. As shown in a previous report, the 17S ribosomal RNA genes (17S rDNA) are located at the nucleolus organizers (NORs) on chromosomes 9 and 10 of the indica cultivar. However, the japonica rice lacked the rDNA signals on chromosome 10. The size of the 5S rDNA repeat block, which was mapped on the chromosome 11 of both cultivars, was 1.22 times larger in the indica than in the japonica genome. The telomeric repeat arrays at the distal ends of all chromosome arms were on average three times longer in the indica genome than in the japonica genome. Flow cytometric measurements revealed that the nuclear DNA content of indica rice is 9.7% higher than that of japonica rice. Our data suggest that different repetitive sequence families contribute significantly to the variation in genome size between indica and japonica rice, though to different extents. The increase or decrease in the copy number of several repetitive sequences examined here may indicate the existence of a directed change in genome size in rice. Possible reasons for this phenomenon of concurrent evolution of various repeat families are discussed.read more
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Journal ArticleDOI
The map-based sequence of the rice genome
Takashi Matsumoto,Jianzhong Wu,Hiroyuki Kanamori,Yuichi Katayose,Masaki Fujisawa,Nobukazu Namiki,Hiroshi Mizuno,Kimiko Yamamoto,Baltazar A. Antonio,Tomoya Baba,Katsumi Sakata,Yoshiaki Nagamura,Hiroyoshi Aoki,Koji Arikawa,Kohei Arita,Takahito Bito,Yoshino Chiden,Nahoko Fujitsuka,Rie Fukunaka,Masao Hamada,Chizuko Harada,Akiko Hayashi,Saori Hijishita,Mikiko Honda,Satomi Hosokawa,Yoko Ichikawa,Atsuko Idonuma,Masumi Iijima,Michiko Ikeda,Maiko Ikeno,Kazue Ito,Sachie Ito,Tomoko Ito,Yuichi Ito,Yukiyo Ito,Aki Iwabuchi,Kozue Kamiya,Wataru Karasawa,Kanako Kurita,Satoshi Katagiri,Ari Kikuta,Harumi Kobayashi,Noriko Kobayashi,Kayo Machita,Tomoko Maehara,Masatoshi Masukawa,Tatsumi Mizubayashi,Yoshiyuki Mukai,Hideki Nagasaki,Yuko Nagata,Shinji Naito,Marina Nakashima,Yuko Nakama,Yumi Nakamichi,Mari Nakamura,Ayano Meguro,Manami Negishi,Isamu Ohta,Tomoya Ohta,Masako Okamoto,Nozomi Ono,Shoko Saji,Miyuki Sakaguchi,Kumiko Sakai,Michie Shibata,Takanori Shimokawa,Jianyu Song,Yuka Takazaki,Kimihiro Terasawa,Mika Tsugane,Kumiko Tsuji,Shigenori Ueda,Kazunori Waki,Harumi Yamagata,Mayu Yamamoto,Shinichi Yamamoto,Hiroko Yamane,Shoji Yoshiki,Rie Yoshihara,Kazuko Yukawa,Huisun Zhong,Masahiro Yano,Takuji Sasaki,Qiaoping Yuan,Shu Ouyang,Jia Liu,Kristine Jones,Kristen Gansberger,Kelly Moffat,Jessica Hill,Jayati Bera,Douglas Fadrosh,Shaohua Jin,Shivani Johri,Mary Kim,Larry Overton,Matthew Reardon,Tamara Tsitrin,Hue Vuong,Bruce Weaver,Anne Ciecko,Luke J. Tallon,Jacqueline Jackson,Grace Pai,Susan Van Aken,T. Utterback,Steve Reidmuller,Tamara Feldblyum,Joseph Hsiao,Victoria Zismann,Stacey E. Iobst,Aymeric R. De Vazeille,C. Robin Buell,Kai Ying,Ying Li,Tingting Lu,Y. Huang,Qiang Zhao,Qi Feng,Lei Zhang,Jingjie Zhu,Qijun Weng,Jie Mu,Yiqi Lu,Danlin Fan,Yilei Liu,Jianping Guan,Yujun Zhang,Shuliang Yu,Xiaohui Liu,Yu Zhang,Guofan Hong,Bin Han,Nathalie Choisne,Nadia Demange,Gisela Orjeda,Sylvie Samain,Laurence Cattolico,Eric Pelletier,Arnaud Couloux,Béatrice Segurens,Patrick Wincker,Angélique D'Hont,Claude Scarpelli,Jean Weissenbach,Marcel Salanoubat,Francis Quetier,Yeisoo Yu,Hyeran Kim,Teri Rambo,Jennifer Currie,Kristi Collura,Meizhong Luo,Tae-Jin Yang,Jetty S.S. Ammiraju,Friedrich Engler,Carol Soderlund,Rod A. Wing,Lance E. Palmer,Melissa De La Bastide,Lori Spiegel,Lidia Nascimento,Theresa Zutavern,A. O'Shaughnessy,Sujit Dike,Neilay Dedhia,R. Preston,Vivekanand Balija,W. Richard McCombie,Teh Yuan Chow,Hong-Hwa Chen,Mei-Chu Chung,Ching San Chen,J. F. Shaw,Hong Pang Wu,Kwang-Jen Hsiao,Ya Ting Chao,Mu Kuei Chu,Chia Hsiung Cheng,Ai Ling Hour,Pei Fang Lee,Shu Jen Lin,Yao-Cheng Lin,John Yu Liou,Shu Mei Liu,Yue-Ie C. Hsing,Saurabh Raghuvanshi,Amitabh Mohanty,Arvind K. Bharti,Arvind K. Bharti,Anupama Gaur,Vipin Gupta,Dibyendu Kumar,Vydianathan Ravi,Shubha Vij,Anita Kapur,Parul Khurana,Paramjit Khurana,Jitendra P. Khurana,Akhilesh K. Tyagi,Kishor Gaikwad,Alok Singh,Vivek Dalal,Subodh K. Srivastava,Anupam Dixit,Ajit K. Pal,Irfan Ahmad Ghazi,Mahavir Yadav,A. Pandit,Atul Bhargava,K. Sureshbabu,Kamlesh Batra,Tilak Raj Sharma,Trilochan Mohapatra,Nagendra K. Singh,Joachim Messing,Amy Bronzino Nelson,Galina Fuks,Steve Kavchok,Gladys Keizer,Eric Linton Victor Llaca,Rentao Song,Bahattin Tanyolac,Steve Young,Kim Yul Ho,Jang Ho Hahn,G. Sangsakoo,Apichart Vanavichit,Luiz Anderson Teixeira de Mattos,Paulo Dejalma Zimmer,Gaspar Malone,Odir Antônio Dellagostin,Antonio Costa de Oliveira,Michael W. Bevan,Ian Bancroft,Patrick Minx,Holly Cordum,Richard K. Wilson,Zhukuan Cheng,Weiwei Jin,Jiming Jiang,Sally A. Leong,Hisakazu Iwama,Takashi Gojobori,Takeshi Itoh,Takeshi Itoh,Yoshihito Niimura,Yasuyuki Fujii,Takuya Habara,Hiroaki Sakai,Hiroaki Sakai,Yoshiharu Sato,Gregory Wilson,Kiran Kumar,Susan R. McCouch,Nikoleta Juretic,Douglas R. Hoen,Stephen I. Wright,Richard Bruskiewich,Thomas E. Bureau,Akio Miyao,Hirohiko Hirochika,Tomotaro Nishikawa,Koh Ichi Kadowaki,Masahiro Sugiura,Benjamin Burr +265 more
TL;DR: A map-based, finished quality sequence that covers 95% of the 389 Mb rice genome, including virtually all of the euchromatin and two complete centromeres, and finds evidence for widespread and recurrent gene transfer from the organelles to the nuclear chromosomes.
Journal ArticleDOI
Improvement of the Oryza sativa Nipponbare reference genome using next generation sequence and optical map data
Yoshihiro Kawahara,Melissa De La Bastide,John P. Hamilton,Hiroyuki Kanamori,W. Richard McCombie,Shu Ouyang,David C. Schwartz,Tsuyoshi Tanaka,Jianzhong Wu,Shiguo Zhou,Kevin L. Childs,Rebecca M. Davidson,Haining Lin,Haining Lin,Lina M. Quesada-Ocampo,Brieanne Vaillancourt,Hiroaki Sakai,Sung Shin Lee,Jungsok Kim,Hisataka Numa,Takeshi Itoh,C. Robin Buell,Takashi Matsumoto +22 more
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Journal ArticleDOI
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Journal ArticleDOI
Current status and the future of fluorescence in situ hybridization (FISH) in plant genome research
TL;DR: It is expected that FISH will continue to play an important role in relating DNA sequence information to chromosome biology and FISH coupled with immunoassays will be increasingly used to study features of chromatin at the cytological level that control expression and regulation of genes.
Journal ArticleDOI
A new protocol for extraction of C0t-1 DNA from rice
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