Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence.
Masashi Narita,Sabrina Nuñez,Sabrina Nuñez,Edith Heard,Masako Narita,Athena W. Lin,Stephen Hearn,David L. Spector,Gregory J. Hannon,Scott W. Lowe +9 more
TLDR
A distinct heterochromatic structure that accumulates in senescent human fibroblasts is described, which is designated senescence-associated heterochROMatic foci (SAHF) and is associated with the stable repression of E2F target genes.About:
This article is published in Cell.The article was published on 2003-06-13 and is currently open access. It has received 2055 citations till now. The article focuses on the topics: Senescence-associated heterochromatin focus & E2F.read more
Citations
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Journal ArticleDOI
RB Reversibly Inhibits DNA Replication via Two Temporally Distinct Mechanisms
Steven P. Angus,Christopher N. Mayhew,David A. Solomon,Wesley A. Braden,Michael P. Markey,Yukiko Okuno,M Christina Cardoso,David M. Gilbert,Erik S. Knudsen +8 more
TL;DR: It is shown that RB does not exert a cis-acting effect on DNA replication, and functional distinctions exist between the “chronic” RB-mediated arrest state and the ”acute” arrest state.
Journal ArticleDOI
miR-29c-3p promotes senescence of human mesenchymal stem cells by targeting CNOT6 through p53-p21 and p16-pRB pathways.
TL;DR: It is demonstrated that miR-29c-3p promotes the senescence of MSCs by targeting CNOT6 through p53-p21 and p16-pRB pathways and highlight the contribution of post-transcriptional regulation to stem cell senescences.
Journal ArticleDOI
The zinc finger transcription factor ZFHX1A is linked to cell proliferation by Rb-E2F1.
Yongqing Liu,Mary E. Costantino,Diego E. Montoya-Durango,Yujiro Higashi,Douglas S. Darling,Douglas C. Dean +5 more
TL;DR: Evidence is provided that ZFHX1A expression is confined to proliferating cells through dependence on the Rb (retinoblastoma protein) family/E2F cell cycle pathway, and it is proposed that the differential effects of the R b family/ E2F pathway on expression of these E-box-binding proteins are important in maintaining their distinct patterns during embryogenesis.
Book ChapterDOI
The role of histone modifications in epigenetic transitions during normal and perturbed development.
Stefan Kubicek,Gunnar Schotta,M. Lachner,Roopsha Sengupta,Alexander Kohlmaier,Laura Perez-Burgos,Y. Linderson,Joost H.A. Martens,Roderick J. O'Sullivan,Barna D. Fodor,Masato Yonezawa,Antoine H.F.M. Peters,Thomas Jenuwein +12 more
TL;DR: In this article, the authors discuss profiles of normal and aberrant histone lysine methylation patterns, as they occur during the transition of an embryonic to a differentiated cell or in controlled self-renewal vs pro-neoplastic or metastatic conditions.
Journal ArticleDOI
SV40 Oncoproteins Enhance Asbestos-Induced DNA Double-Strand Breaks and Abrogate Senescence in Murine Mesothelial Cells
Jodie R. Pietruska,Agnes B. Kane +1 more
TL;DR: SV40 virus may contribute to mesothelioma progression by impairing stress-induced senescence, in part through p53 inactivation, thereby favoring survival and proliferation of mesothelial cells that have sustained DNA damage.
References
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Journal ArticleDOI
A biomarker that identifies senescent human cells in culture and in aging skin in vivo
Goberdhan P. Dimri,X Lee,G Basile,Meileen Acosta,G Scott,C Roskelley,E E Medrano,Maarten H.K. Linskens,Ivica Rubelj,Olivia M. Pereira-Smith +9 more
TL;DR: It is shown that several human cells express a beta-galactosidase, histochemically detectable at pH 6, upon senescence in culture, which provides in situ evidence that senescent cells may exist and accumulate with age in vivo.
Journal ArticleDOI
The limited in vitro lifetime of human diploid cell strains
TL;DR: The survival curves obtained with human diploid cell strains are comparable to “multiple-hit” or “ multiple-target” curves obtain with other biological systems where an initial threshold dose is required before an exponential form of the curve is established.
Journal ArticleDOI
Oncogenic ras Provokes Premature Cell Senescence Associated with Accumulation of p53 and p16INK4a
TL;DR: It is shown that expression of oncogenic ras in primary human or rodent cells results in a permanent G1 arrest, and that the onset of cellular senescence does not simply reflect the accumulation of cell divisions, but can be prematurely activated in response to an onCogenic stimulus.
Journal ArticleDOI
Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins.
TL;DR: It is shown that mammalian methyltransferases that selectively methylate histone H3 on lysine 9 (Suv39h HMTases) generate a binding site for HP1 proteins—a family of heterochromatic adaptor molecules implicated in both gene silencing and supra-nucleosomal chromatin structure.
Journal ArticleDOI
Selective recognition of methylated lysine 9 on histone H3 by the HP1 chromo domain.
Andrew J. Bannister,Philip Zegerman,Janet F. Partridge,Eric A. Miska,Jean O. Thomas,Robin C. Allshire,Tony Kouzarides +6 more
TL;DR: A stepwise model for the formation of a transcriptionally silent heterochromatin is provided: SUV39H1 places a ‘methyl marker’ on histone H3, which is then recognized by HP1 through its chromo domain, which may also explain the stable inheritance of theheterochromatic state.