Journal ArticleDOI
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
TLDR
The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns, has been designed and used to expand the domain of embryonic expression of the homeobox protein even-skipped.Abstract:
We have designed a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. The gene encoding the yeast transcriptional activator GAL4 is inserted randomly into the Drosophila genome to drive GAL4 expression from one of a diverse array of genomic enhancers. It is then possible to introduce a gene containing GAL4 binding sites within its promoter, to activate it in those cells where GAL4 is expressed, and to observe the effect of this directed misexpression on development. We have used GAL4-directed transcription to expand the domain of embryonic expression of the homeobox protein even-skipped. We show that even-skipped represses wingless and transforms cells that would normally secrete naked cuticle into denticle secreting cells. The GAL4 system can thus be used to study regulatory interactions during embryonic development. In adults, targeted expression can be used to generate dominant phenotypes for use in genetic screens. We have directed expression of an activated form of the Dras2 protein, resulting in dominant eye and wing defects that can be used in screens to identify other members of the Dras2 signal transduction pathway.read more
Citations
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Journal ArticleDOI
Analysis of Dscam Diversity in Regulating Axon Guidance in Drosophila Mushroom Bodies
Xiao Li Zhan,James C. Clemens,Guilherme Neves,Daisuke Hattori,John J. Flanagan,Thomas Hummel,M. Luisa Vasconcelos,Andrew Chess,S. Lawrence Zipursky +8 more
TL;DR: It is proposed that different extracellular domains of Dscam share a common function and that differences in isoforms expressed on the surface of neighboring axons influence interactions between them.
Journal ArticleDOI
The Drosophila Hox Gene Deformed Sculpts Head Morphology via Direct Regulation of the Apoptosis Activator reaper
TL;DR: During early Drosophila development, the Hox protein Deformed maintains the boundary between the maxillary and mandibular head lobes by activating localized apoptosis, which literally sculpts segments into distinct shapes.
Journal ArticleDOI
Group choreography: mechanisms orchestrating the collective movement of border cells
TL;DR: An overview of the molecular choreography of border cells of the Drosophila melanogaster ovary is provided and its more general implications are provided.
Journal ArticleDOI
Neuromuscular defects in a Drosophila survival motor neuron gene mutant
Yick-Bun Chan,Irene Miguel-Aliaga,Chris Franks,Natasha Thomas,Barbara Trülzsch,David B. Sattelle,Kay E. Davies,Marcel van den Heuvel +7 more
TL;DR: A new Drosophila model for SMA is proposed, finding that zygotic smn mutant animals show abnormal motor behavior and that smn gene activity is required in both neurons and muscle to alleviate this phenotype, and proposes a functional role for SMN at the neuromUScular junction in the generation of neuromuscular defects.
Journal ArticleDOI
The Drosophila heterochromatic gene encoding poly(ADP-ribose) polymerase (PARP) is required to modulate chromatin structure during development
TL;DR: It is proposed that PARP-e autoregulates Parp transcription by influencing the chromatin structure of its heterochromatic environment and indicates that Parp plays a fundamental role organizing the structure of Drosophila chromatin.
References
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Book
The Genome of Drosophila Melanogaster
TL;DR: Chromosomes: Deficiencies, Inversions, and Transposable Elements.
Book
The Embryonic Development of Drosophila melanogaster
TL;DR: Stages of Drosophila Embryogenesis, some Aspects of Segmentation, and a Fate Map of the Blastoderm are described.
Journal ArticleDOI
How eukaryotic transcriptional activators work
TL;DR: A specific protein, bound to DNA, can activate transcription of a wide array of genes in many eukaryotes and is controlled by the immune system.
Journal ArticleDOI
A stable genomic source of P element transposase in Drosophila melanogaster.
Hugh M. Robertson,Christine R. Preston,Randall W. Phillis,Dena M. Johnson-Schlitz,Wendy K. Benz,William R. Engels +5 more
TL;DR: A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable.
Book
Drosophila: A Practical Approach
TL;DR: The localisation of RNAs in drosophila tissue sections by in situ hybridisation injecting eggs P element-mediated transformation and Methods of marking cells Cell surface antigens Preparation of nucleic acids.
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