Journal ArticleDOI
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
TLDR
The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns, has been designed and used to expand the domain of embryonic expression of the homeobox protein even-skipped.Abstract:
We have designed a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. The gene encoding the yeast transcriptional activator GAL4 is inserted randomly into the Drosophila genome to drive GAL4 expression from one of a diverse array of genomic enhancers. It is then possible to introduce a gene containing GAL4 binding sites within its promoter, to activate it in those cells where GAL4 is expressed, and to observe the effect of this directed misexpression on development. We have used GAL4-directed transcription to expand the domain of embryonic expression of the homeobox protein even-skipped. We show that even-skipped represses wingless and transforms cells that would normally secrete naked cuticle into denticle secreting cells. The GAL4 system can thus be used to study regulatory interactions during embryonic development. In adults, targeted expression can be used to generate dominant phenotypes for use in genetic screens. We have directed expression of an activated form of the Dras2 protein, resulting in dominant eye and wing defects that can be used in screens to identify other members of the Dras2 signal transduction pathway.read more
Citations
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Journal ArticleDOI
Drosophila Fragile X-Related Gene Regulates the MAP1B Homolog Futsch to Control Synaptic Structure and Function
Yong Q. Zhang,Adina M. Bailey,Heinrich J.G. Matthies,Robert Renden,Mark A. Smith,Sean D. Speese,Gerald M. Rubin,Kendal Broadie +7 more
TL;DR: It is proposed that dFXR acts as a translational repressor of Futsch to regulate microtubule-dependent synaptic growth and function.
Journal ArticleDOI
Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics.
Jasper Akerboom,Nicole Carreras Calderón,Nicole Carreras Calderón,Nicole Carreras Calderón,Lin Tian,Lin Tian,Sebastian Wabnig,Matthias Prigge,Johan Tolö,Andrew Gordus,Michael B. Orger,Michael B. Orger,Kristen E. Severi,John J. Macklin,Ronak Patel,Stefan R. Pulver,Trevor J. Wardill,Trevor J. Wardill,Elisabeth Fischer,Christina Schüler,Tsai Wen Chen,Karen S. Sarkisyan,Jonathan S. Marvin,Cornelia I. Bargmann,Douglas S. Kim,Sebastian Kügler,Leon Lagnado,Peter Hegemann,Alexander Gottschalk,Eric R. Schreiter,Eric R. Schreiter,Loren L. Looger +31 more
TL;DR: Red, single-wavelength GECIs, “RCaMPs,” engineered from circular permutation of the thermostable red fluorescent protein mRuby are described and 2-color calcium imaging is demonstrated both within the same cell (registering mitochondrial and somatic [Ca2+]) and between two populations of cells: neurons and astrocytes.
Journal ArticleDOI
Expanded Polyglutamine Protein Forms Nuclear Inclusions and Causes Neural Degeneration in Drosophila
John M. Warrick,Henry L. Paulson,Gladys L. Gray-Board,Quang T. Bui,Kenneth H. Fischbeck,Randall N. Pittman,Nancy M. Bonini +6 more
TL;DR: The results demonstrate that cellular mechanisms of human glutamine-repeat disease are conserved in invertebrates and will aid in identifying additional factors that modulate neurodegeneration.
Journal ArticleDOI
The art and design of genetic screens: Drosophila melanogaster
TL;DR: The ingenious approaches that have been devised to circumvent traditional screens now make it possible to screen for almost any phenotype in any cell at any stage of development.
Journal ArticleDOI
Identification of genes that modify ataxin-1-induced neurodegeneration
Pedro Fernandez-Funez,Maria Laura Nino-Rosales,Beatrice De Gouyon,Wei Chi She,James M. Luchak,Pedro Martinez,Enrique Turiegano,Jonathan Benito,Maria Capovilla,Pamela J. Skinner,Alanna E. McCall,Inmaculada Canal,Harry T. Orr,Huda Y. Zoghbi,Juan Botas +14 more
TL;DR: It is shown that high levels of wild-type ataxin-1 can cause degenerative phenotypes similar to those caused by the expanded protein, and may be relevant to the treatment of polyglutamine diseases and, perhaps, to other neurodegenerative diseases, such as Alzheimer's and Parkinson's disease.
References
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Book
The Genome of Drosophila Melanogaster
TL;DR: Chromosomes: Deficiencies, Inversions, and Transposable Elements.
Book
The Embryonic Development of Drosophila melanogaster
TL;DR: Stages of Drosophila Embryogenesis, some Aspects of Segmentation, and a Fate Map of the Blastoderm are described.
Journal ArticleDOI
How eukaryotic transcriptional activators work
TL;DR: A specific protein, bound to DNA, can activate transcription of a wide array of genes in many eukaryotes and is controlled by the immune system.
Journal ArticleDOI
A stable genomic source of P element transposase in Drosophila melanogaster.
Hugh M. Robertson,Christine R. Preston,Randall W. Phillis,Dena M. Johnson-Schlitz,Wendy K. Benz,William R. Engels +5 more
TL;DR: A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable.
Book
Drosophila: A Practical Approach
TL;DR: The localisation of RNAs in drosophila tissue sections by in situ hybridisation injecting eggs P element-mediated transformation and Methods of marking cells Cell surface antigens Preparation of nucleic acids.
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