Journal ArticleDOI
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
TLDR
The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns, has been designed and used to expand the domain of embryonic expression of the homeobox protein even-skipped.Abstract:
We have designed a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. The gene encoding the yeast transcriptional activator GAL4 is inserted randomly into the Drosophila genome to drive GAL4 expression from one of a diverse array of genomic enhancers. It is then possible to introduce a gene containing GAL4 binding sites within its promoter, to activate it in those cells where GAL4 is expressed, and to observe the effect of this directed misexpression on development. We have used GAL4-directed transcription to expand the domain of embryonic expression of the homeobox protein even-skipped. We show that even-skipped represses wingless and transforms cells that would normally secrete naked cuticle into denticle secreting cells. The GAL4 system can thus be used to study regulatory interactions during embryonic development. In adults, targeted expression can be used to generate dominant phenotypes for use in genetic screens. We have directed expression of an activated form of the Dras2 protein, resulting in dominant eye and wing defects that can be used in screens to identify other members of the Dras2 signal transduction pathway.read more
Citations
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Journal ArticleDOI
Dissection of memory formation: from behavioral pharmacology to molecular genetics
Jim DeZazzo,Tim Tully +1 more
TL;DR: These studies support the application of reverse-genetic strategies, including the use of temporally specific agonists and antagonists, to advance the functional dissection of memory formation.
Journal ArticleDOI
Electrical Hyperexcitation of Lateral Ventral Pacemaker Neurons Desynchronizes Downstream Circadian Oscillators in the Fly Circadian Circuit and Induces Multiple Behavioral Periods
Michael N. Nitabach,Ying Wu,Vasu Sheeba,William C. Lemon,John G. Strumbos,Paul K. Zelensky,Benjamin H. White,Todd C. Holmes +7 more
TL;DR: Regulated electrical activity of the LNVs synchronize multiple oscillators in the fly circadian pacemaker circuit and renders them hyperexcitable via transgenic expression of a low activation threshold voltage-gated sodium channel.
Journal ArticleDOI
VRILLE feeds back to control circadian transcription of Clock in the Drosophila circadian oscillator
TL;DR: It is shown that a CLK-CYC-activated gene, vrille (vri), encodes a repressor of Clk transcription, thereby identifying vri as a key negative component of the Clk feedback loop in Drosophila's circadian oscillator.
Journal ArticleDOI
Gene expression systems in Drosophila: a synthesis of time and space.
TL;DR: Progress is reviewed on the development and implementation of several gene expression systems that offer control in time and space and systems employing the yeast FLP recombinase gene and FRT sites are reviewed.
Journal ArticleDOI
A Drosophila model for LRRK2-linked parkinsonism
Zhaohui Liu,Xiaoyue Wang,Yi Yu,Xueping Li,Tao Wang,Haibing Jiang,Qiuting Ren,Yuchen Jiao,Akira Sawa,Timothy H. Moran,Christopher A. Ross,Craig Montell,Wanli W. Smith +12 more
TL;DR: This is the first in vivo“gain-of-function” model which recapitulates several key features of LRRK2-linked human parkinsonism and may provide a useful model for studying L RRK 2-linked pathogenesis and for future therapeutic screens for PD intervention.
References
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Book
The Genome of Drosophila Melanogaster
TL;DR: Chromosomes: Deficiencies, Inversions, and Transposable Elements.
Book
The Embryonic Development of Drosophila melanogaster
TL;DR: Stages of Drosophila Embryogenesis, some Aspects of Segmentation, and a Fate Map of the Blastoderm are described.
Journal ArticleDOI
How eukaryotic transcriptional activators work
TL;DR: A specific protein, bound to DNA, can activate transcription of a wide array of genes in many eukaryotes and is controlled by the immune system.
Journal ArticleDOI
A stable genomic source of P element transposase in Drosophila melanogaster.
Hugh M. Robertson,Christine R. Preston,Randall W. Phillis,Dena M. Johnson-Schlitz,Wendy K. Benz,William R. Engels +5 more
TL;DR: A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable.
Book
Drosophila: A Practical Approach
TL;DR: The localisation of RNAs in drosophila tissue sections by in situ hybridisation injecting eggs P element-mediated transformation and Methods of marking cells Cell surface antigens Preparation of nucleic acids.
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