Journal ArticleDOI
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
TLDR
The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns, has been designed and used to expand the domain of embryonic expression of the homeobox protein even-skipped.Abstract:
We have designed a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. The gene encoding the yeast transcriptional activator GAL4 is inserted randomly into the Drosophila genome to drive GAL4 expression from one of a diverse array of genomic enhancers. It is then possible to introduce a gene containing GAL4 binding sites within its promoter, to activate it in those cells where GAL4 is expressed, and to observe the effect of this directed misexpression on development. We have used GAL4-directed transcription to expand the domain of embryonic expression of the homeobox protein even-skipped. We show that even-skipped represses wingless and transforms cells that would normally secrete naked cuticle into denticle secreting cells. The GAL4 system can thus be used to study regulatory interactions during embryonic development. In adults, targeted expression can be used to generate dominant phenotypes for use in genetic screens. We have directed expression of an activated form of the Dras2 protein, resulting in dominant eye and wing defects that can be used in screens to identify other members of the Dras2 signal transduction pathway.read more
Citations
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Journal ArticleDOI
Absence of effects of Sir2 overexpression on lifespan in C. elegans and Drosophila
Camilla Burnett,Sara Valentini,Filipe Cabreiro,Martin Goss,Milán Somogyvári,Matthew D.W. Piper,Matthew P. Hoddinott,George L. Sutphin,Vid Leko,Joshua J McElwee,Rafael P. Vázquez-Manrique,Rafael P. Vázquez-Manrique,Anne-Marie Orfila,Anne-Marie Orfila,Daniel Ackerman,Catherine Au,Giovanna Vinti,Michele Riesen,Ken Howard,Christian Neri,Christian Neri,Antonio Bedalov,Matt Kaeberlein,Csaba Soti,Linda Partridge,David Gems +25 more
TL;DR: These findings cast doubt on the robustness of the previously reported effects of sirtuins on lifespan in C. elegans and Drosophila and underscore the importance of controlling for genetic background and for the mutagenic effects of transgene insertions in studies of genetic effects on lifespan.
Journal ArticleDOI
Requirement for a peptidoglycan recognition protein (PGRP) in Relish activation and antibacterial immune responses in Drosophila.
Kwang Min Choe,Kwang Min Choe,Thomas Werner,Svenja Stöven,Dan Hultmark,Kathryn V. Anderson,Kathryn V. Anderson +6 more
TL;DR: It is shown that a peptidoglycan recognition protein, PGRP-LC, is absolutely required for the induction of antibacterial peptide genes in response to infection in Drosophila and acts by controlling activation of the NF-κB family transcription factor Relish.
Journal ArticleDOI
Fringe Modulates Notch-ligand Interactions
TL;DR: The Notch family of transmembrane receptor proteins mediate developmental cell-fate decisions, and mutations in mammalian Notch genes have been implicated in leukaemia, breast cancer, stroke and dementia.
Journal ArticleDOI
Genetic Suppression of Polyglutamine Toxicity in Drosophila
TL;DR: Two suppressor strains were isolated among 7000 P-element insertions that led to the discovery of the suppressor genes described here, which contain a chaperone-related J domain and suppression of polyglutamine toxicity was verified in transgenic flies.
Journal ArticleDOI
Spatial Representation of the Glomerular Map in the Drosophila Protocerebrum
TL;DR: In the fruit fly, Drosophila, olfactory sensory neurons expressing a given receptor project to spatially invariant loci in the antennal lobe to create a topographic map of receptor activation, which is represented in higher sensory centers in the brain by projection neurons that innervate the same glomerulus.
References
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Book
The Genome of Drosophila Melanogaster
TL;DR: Chromosomes: Deficiencies, Inversions, and Transposable Elements.
Book
The Embryonic Development of Drosophila melanogaster
TL;DR: Stages of Drosophila Embryogenesis, some Aspects of Segmentation, and a Fate Map of the Blastoderm are described.
Journal ArticleDOI
How eukaryotic transcriptional activators work
TL;DR: A specific protein, bound to DNA, can activate transcription of a wide array of genes in many eukaryotes and is controlled by the immune system.
Journal ArticleDOI
A stable genomic source of P element transposase in Drosophila melanogaster.
Hugh M. Robertson,Christine R. Preston,Randall W. Phillis,Dena M. Johnson-Schlitz,Wendy K. Benz,William R. Engels +5 more
TL;DR: A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable.
Book
Drosophila: A Practical Approach
TL;DR: The localisation of RNAs in drosophila tissue sections by in situ hybridisation injecting eggs P element-mediated transformation and Methods of marking cells Cell surface antigens Preparation of nucleic acids.
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