Journal ArticleDOI
Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
Andrea H. Brand,Norbert Perrimon +1 more
TLDR
The GAL4 system, a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns, has been designed and used to expand the domain of embryonic expression of the homeobox protein even-skipped.Abstract:
We have designed a system for targeted gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. The gene encoding the yeast transcriptional activator GAL4 is inserted randomly into the Drosophila genome to drive GAL4 expression from one of a diverse array of genomic enhancers. It is then possible to introduce a gene containing GAL4 binding sites within its promoter, to activate it in those cells where GAL4 is expressed, and to observe the effect of this directed misexpression on development. We have used GAL4-directed transcription to expand the domain of embryonic expression of the homeobox protein even-skipped. We show that even-skipped represses wingless and transforms cells that would normally secrete naked cuticle into denticle secreting cells. The GAL4 system can thus be used to study regulatory interactions during embryonic development. In adults, targeted expression can be used to generate dominant phenotypes for use in genetic screens. We have directed expression of an activated form of the Dras2 protein, resulting in dominant eye and wing defects that can be used in screens to identify other members of the Dras2 signal transduction pathway.read more
Citations
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Journal ArticleDOI
Evidence for a Growth-Stabilizing Regulatory Feedback Mechanism between Myc and Yorkie, the Drosophila Homolog of Yap
TL;DR: It is proposed that the codependent regulatory relationship functionally coordinates the cellular activities of Yki and Myc and provides a mechanism of growth control that regulates organ size and has broad implications for cancer.
Journal ArticleDOI
Generation of multiple antagonistic domains along the proximodistal axis during Drosophila leg development
Muna Abu-Shaar,Richard S. Mann +1 more
TL;DR: There exists in the leg disc a set of mutually antagonistic interactions between proximal cells, which are suggested to convert the WG and DPP activity gradients into discreet domains of gene expression along the proximodistal axis.
Journal ArticleDOI
Smaug recruits the CCR4/POP2/NOT deadenylase complex to trigger maternal transcript localization in the early Drosophila embryo.
Jennifer L. Semotok,Ramona L. Cooperstock,Benjamin D Pinder,Heli K. Vari,Howard D. Lipshitz,Craig A. Smibert +5 more
TL;DR: This work identifies the RNA binding protein, Smaug, previously known as a translational repressor of nanos, as a key regulator of degradation/protection-based transcript localization, a multifunctional posttranscriptional regulator that employs distinct mechanisms to repress translation and to induce degradation of target transcripts.
Journal ArticleDOI
The bantam MicroRNA Is a Target of the Hippo Tumor-Suppressor Pathway
Riitta Nolo,Clayton M. Morrison,Clayton M. Morrison,Chunyao Tao,Xinwei Zhang,Georg Halder,Georg Halder +6 more
TL;DR: It is reported that the bantam microRNA is a downstream target of the Hippo signaling pathway and that hippo mutant cells had elevated levels of bantam activity and that bantam was required for Yorkie-driven overgrowth.
Journal ArticleDOI
Regulation of hunger-driven behaviors by neural ribosomal S6 kinase in Drosophila.
Qi Wu,Yan Zhang,Jie Xu,Ping Shen +3 more
TL;DR: It is found that overexpression of Drosophila insulin-like peptides in the nervous system of fasted larvae suppressed the hunger-driven increase of ingestion rate and intake of nonpreferred foods.
References
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Book
The Genome of Drosophila Melanogaster
TL;DR: Chromosomes: Deficiencies, Inversions, and Transposable Elements.
Book
The Embryonic Development of Drosophila melanogaster
TL;DR: Stages of Drosophila Embryogenesis, some Aspects of Segmentation, and a Fate Map of the Blastoderm are described.
Journal ArticleDOI
How eukaryotic transcriptional activators work
TL;DR: A specific protein, bound to DNA, can activate transcription of a wide array of genes in many eukaryotes and is controlled by the immune system.
Journal ArticleDOI
A stable genomic source of P element transposase in Drosophila melanogaster.
Hugh M. Robertson,Christine R. Preston,Randall W. Phillis,Dena M. Johnson-Schlitz,Wendy K. Benz,William R. Engels +5 more
TL;DR: A single P element insert in Drosophila melanogaster, called P[ry+ delta 2-3](99B), is described that caused mobilization of other elements at unusually high frequencies, yet is itself remarkably stable.
Book
Drosophila: A Practical Approach
TL;DR: The localisation of RNAs in drosophila tissue sections by in situ hybridisation injecting eggs P element-mediated transformation and Methods of marking cells Cell surface antigens Preparation of nucleic acids.
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