Why does insect RNA look degraded
TLDR
The electrophoretic rRNA profile of insects, using the honey bee, Apis mellifera, as an example, differs significantly from the standard benchmark since the 28S rRNA of most insects contains an endogenous “hidden break.”Abstract:
The integrity of extracted ribonucleic acid (RNA) is commonly assessed by gel electrophoresis and subsequent analysis of the ribosomal RNA (rRNA) bands. Using the honey bee, Apis mellifera (Hymenoptera: Apidae), as an example, the electrophoretic rRNA profile of insects is explained. This profile differs significantly from the standard benchmark since the 28S rRNA of most insects contains an endogenous “hidden break.” Upon denaturation, the masking hydrogen bonds are disrupted, releasing two similar sized fragments that both migrate closely with 18S rRNA. The resulting rRNA profile thus reflects the endogenous composition of insect rRNA and should not be misinterpreted as degradation.read more
Citations
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Miscellaneous standard methods for Apis mellifera research
Hannelie Human,Robert Brodschneider,Vincent Dietemann,Galen P. Dively,James D. Ellis,Eva Forsgren,Ingemar Fries,Fani Hatjina,Fuliang Hu,Rodolfo Jaffé,Annette Bruun Jensen,Angela Köhler,Josef P Magyar,Asli Özkýrým,Christian Walter Werner Pirk,Robyn Rose,Ursula Strauss,Gina Tanner,David R. Tarpy,Jozef J. M. van der Steen,Anthony D. Vaudo,Fleming Vejsnæs,Jerzy Wilde,Geoffrey R. Williams,Huoqing Zheng +24 more
TL;DR: These methods, described in this paper, are especially valuable when investigating the effects of pesticide applications, environmental pollution and diseases on colony survival.
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Adaptations to a subterranean environment and longevity revealed by the analysis of mole rat genomes
Xiaodong Fang,Inge Seim,Zhiyong Huang,Maxim V. Gerashchenko,Zhiqiang Xiong,Anton A. Turanov,Yabing Zhu,Alexei V. Lobanov,Dingding Fan,Sun Hee Yim,Xiaoming Yao,Siming Ma,Lan Yang,Sang-Goo Lee,Sang-Goo Lee,Eun Bae Kim,Roderick T. Bronson,Radim Šumbera,Rochelle Buffenstein,Xin Zhou,Anders Krogh,Thomas J. Park,Guojie Zhang,Jun Wang,Jun Wang,Vadim N. Gladyshev,Vadim N. Gladyshev +26 more
TL;DR: The Damaraland mole rat (DMR, Fukomys damarensis) and NMR-enhanced naked mole rat were compared in this article, revealing a divergent insulin peptide, expression of oxygen-carrying globins in the brain, prevention of high CO2induced pain perception, and enhanced ammonia detoxification.
Journal ArticleDOI
Looking for reference genes for real-time quantitative PCR experiments in Rhodnius prolixus (Hemiptera: Reduviidae).
David Majerowicz,Michele Alves-Bezerra,Raquel Logullo,André Luiz Fonseca-de-Souza,José Roberto Meyer-Fernandes,Glória R.C. Braz,Katia C. Gondim +6 more
TL;DR: Rp18S and elongation factor 1 (RpEF ‐1) were the most reliable genes for normalization in qPCR when gene expression in different organs was compared and both genes were found to be the best references when transcript levels were compared in the posterior midgut of insects infected with Trypanosoma cruzi.
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Molecular and functional characterization of CYP6BQ23, a cytochrome P450 conferring resistance to pyrethroids in European populations of pollen beetle, Meligethes aeneus.
Christoph T. Zimmer,Chris Bass,Martin S. Williamson,Martin Kaussmann,Katharina Wölfel,Oliver Gutbrod,Ralf Nauen +6 more
TL;DR: A single P450 gene, CYP6BQ23, which is significantly and highly overexpressed in adults and larvae of pyrethroid resistant strains compared to susceptible strains is revealed and results strongly suggest that the overexpression of CYP 6BQ 23 is the primary mechanism conferring pyrethoid resistance in pollen beetle populations throughout much of Europe.
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The Global Invertebrate Genomics Alliance (GIGA): developing community resources to study diverse invertebrate genomes
TL;DR: The intent is to build a collaborative network of diverse scientists to tackle major challenges associated with genome/transcriptome sequencing across a large taxonomic spectrum and to promote standards that will facilitate comparative approaches to invertebrate genomics and collaborations across the international scientific community.
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