Bharathiar University

About: Bharathiar University is a education organization based out in Coimbatore, Tamil Nadu, India. It is known for research contribution in the topics: Thin film & Adsorption. The organization has 5812 authors who have published 8628 publications receiving 143934 citations. The organization is also known as: BU.

Preparation, characterization, in-vitro drug release and cellular uptake of poly(caprolactone) grafted dextran copolymeric nanoparticles loaded with anticancer drug.

Abstract: Biodegradable and biocompatible polymers that are engineered to nanostructures play a key role in providing solution for sustained chemotherapy. This study is focused on preparation, drug encapsulation efficiency, in-vitro drug release, in-vitro cellular uptake and cell viability of poly(caprolactone) grafted dextran (PGD) nanoparticles (NPs) formulation containing vinblastine as the anticancer drug. Drug-loaded PGD NPs were prepared by a modified oil/water emulsion method and characterized by laser light scattering, atomic force microscopy (AFM), and zeta potential. The drug encapsulation efficiency was determined spectrophotometrically and in-vitro drug release was estimated using dialysis bag. Breast cancer cell line (MCF-7) was used to image and measure the cellular uptake of fluorescent PGD NPs. Cancer cell viability was assessed by treating MCF-7 cells with vinblastine-loaded PGD NPs by crystal violet staining method. Result showed that the vinblastine-loaded PGD NPs were superior in properties such as drug encapsulation efficiency, the cellular uptake and the cancer cell mortality.

Synergistic effect of HIF-1α and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition.

Abstract: Cardiomyocyte death is an important pathogenic feature of ischemia and heart failure. Through this study, we showed the synergistic role of HIF-1α and FoxO3a in cardiomyocyte apoptosis subjected to hypoxia plus elevated glucose levels. Using gene specific small interfering RNAs (siRNA), semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, nuclear and cytosolic localization and TUNEL assay techniques, we determined that combined function of HIF-1α and FoxO3a under high glucose plus hypoxia condition lead to enhanced expression of BNIP3 inducing cardiomyocyte death. Our results highlighted the importance of the synergistic role of HIF-1α and FoxO3a in cardiomyocyte death which may add insight into therapeutic approaches to pathophysiology associated with ischemic diabetic cardiomyopathies.

Prostaglandin E2-Induced COX-2 Expressions via EP2 and EP4 Signaling Pathways in Human LoVo Colon Cancer Cells.

Abstract: Metastasis is the most dangerous risk faced by patients with hereditary non-polyposis colon cancer (HNPCC) The expression of matrix metalloproteinases (MMPs) has been observed in several types of human cancers and regulates the efficacy of many therapies Here, we show that treatment with various concentrations of prostaglandin E2 (PGE2; 0, 1, 5 or 10 μM) promotes the migration ability of the human LoVo colon cancer cell line As demonstrated by mRNA and protein expression analyses, EP2 and EP4 are the major PGE2 receptors expressed on the LoVo cell membrane The Phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K)/Akt cell survival pathway was upregulated by EP2 and EP4 activation Following the activation of the PI3K/Akt pathway, β-catenin translocated into the nucleus and triggered COX2 transcription via LEF-1 and TCF-4 and its subsequent translation COX2 expression correlated with the elevation in the migration ability of LoVo cells The experimental evidence shows a possible mechanism by which PGE2 induces cancer cell migration and further suggests PGE2 to be a potential therapeutic target in colon cancer metastasis On inhibition of PGE2, in order to determine the downstream pathway, the levels of PI3K/Akt pathway were suppressed and the β-catenin expression was also modulated Inhibition of EP2 and EP4 shows that PGE2 induces protein expression of COX-2 through EP2 and EP4 receptors in LoVo colon cancer cells

Simple and visual approach for highly selective biosensing of vitamin B1 based on glutathione coated silver nanoparticles as a colorimetric probe

Abstract: We report a simple visual colorimetric method for the highly sensitive and selective detection of thiamine (Vitamin B1) by silver nanoparticles (AgNPs) as a probe. AgNPs surface was modified by glutathione (GSH) and their morphology, size distribution and optical properties were characterized by high resolution transmission electron microscope (HR-TEM) and various analytical spectroscopic techniques. The sensitivity of colorimetric assay is based on the surface plasmon resonance band (SPR) changes and aggregation of glutathione modified AgNPs (GSH-AgNPs) upon the successive addition of Vitamin B1. The selectivity of GSH-AgNPs based determination of Vitamin B1 is excellent, compared with other relevant vitamin interferences. GSH-AgNPs based sensor capably and specifically identified for the detection of Vitamin B1, it caused by their ability to form strong electrostatic interaction between positively charged Vitamin B1 and negatively charged GSH coated AgNPs. This spectral and visual colorimetric approach illustrates better sensitivity for Vitamin B1 with a lowest detection limit reaches 50.00 × 10 −9 mol dm −3 . To establish the practical applications of the GSH-AgNPs probe in real sample analysis, the colorimetric assay was effectively applied to the detection of Vitamin B1 in spiked human blood serum and urine samples.