Henan University of Technology

About: Henan University of Technology is a education organization based out in Zhengzhou, China. It is known for research contribution in the topics: Catalysis & Chemistry. The organization has 7648 authors who have published 6503 publications receiving 73067 citations. The organization is also known as: Hénán Gōngyè Dàxué.

Notice of Retraction Review of decision trees

Abstract: The decision tree algorithm is a hot point in the field of data mining, which is usually used to form classifiers and prediction models. In practice, it has a wide application. This paper describes the decision tree technology and its development process, focuses on typical decision tree algorithms, analyzes their advantages and disadvantages, compares several algorithms, and finally discusses the development of the decision tree algorithm in the future.

Identification and Characterization of MicroRNAs in the Spleen of Common Carp Immune Organ

Abstract: MicroRNAs (miRNAs) play an important role in the regulation of many fundamental biological processes in eukaryotes; however, miRNAs associated with immune functions in the common carp have not been reported. In this study, a small-RNA cDNA library was constructed from the spleen of the common carp. A total of 10,603,456 high-quality clean reads, representing 293,603 unique sequences, were obtained from the small-cDNA library using the Solexa sequencing. By the bioinformatic analysis, 194 conserved miRNAs and 12 novel miRNAs were identified in the carp spleen. The abundant miRNAs principally belong to 30 miRNA gene families such as let-7, mir-10, mir-15, mir-30, and so on. The conservation analysis showed that 23 families were present both in protostomes and deuterostomes, 46 families were conserved only in vertebrates, and 5 families (mir-430, mir-722, mir-724, mir-734, and mir-738) were identified only in fish species. Furthermore, GO enrichment analysis and KEGG pathway analysis suggested that miRNAs expressed in the spleen of common carp are involved in immune system development, lymphoid organ development, lymphocyte activation, immune response, B cell receptor signaling pathway, T cell receptor signaling pathway, Fc gamma R-mediated phagocytosis, Toll-like receptor signaling pathway, and so on. This study described the miRNA transcriptome in spleen tissue for the first time in the common carp. The results expand the number of known common carp miRNAs and provides a meaningful framework to understand the common carp immune system and defense mechanisms.

Production of a recombinant carrot antifreeze protein by Pichia pastoris GS115 and its cryoprotective effects on frozen dough properties and bread quality

Abstract: Carrot (Daucus carota) antifreeze protein (CaAFP) is reported to have strong ice recrystallization inhibition (IRI) activity and great potential in frozen dough. However, the low yield and high cost of extracting CaAFP from natural sources limit its wide applications. To overcome the paucity of CaAFP, in this study, recombinant carrot antifreeze protein (rCaAFP) was expressed and produced in Pichia pastoris GS115, and the cryoprotective effects of rCaAFP on frozen dough properties and bread quality were investigated. The rCaAFP production reached 379.47 mg/L in a 7 L fermentor, and the rCaAFP exhibited high thermal hysteresis activity (1.96 °C at 10 mg/mL) and strong IRI activity. The addition of rCaAFP to frozen dough lessened the increment of freezable water content during freeze-thawed cycles, weakened the damage of freeze-thawed cycles to fermentation capacity and microstructure of frozen dough, and consequently improved the specific volume and texture properties of bread. The rCaAFP produced by Pichia pastoris GS115 was proven to be an effective cryoprotectant for frozen dough. This study provides a feasible method for the production of recombinant plant antifreeze proteins. The availability of gram quantities of rCaAFP contributes to accelerating further application studies.