Henan University of Technology

About: Henan University of Technology is a education organization based out in Zhengzhou, China. It is known for research contribution in the topics: Catalysis & Chemistry. The organization has 7648 authors who have published 6503 publications receiving 73067 citations. The organization is also known as: Hénán Gōngyè Dàxué.

Overexpression of a Wheat CCaMK Gene Reduces ABA Sensitivity of Arabidopsis thaliana During Seed Germination and Seedling Growth

Abstract: Abscisic acid (ABA) plays pivotal roles in plant biotic and abiotic stress responses, where calcium ions are important second messengers. Calcium/calmodulin-dependent protein kinase (CCaMK) is essential for nodulation in legumes, but whether it will perceive calcium signals from abiotic stresses is not clear, especially in non-legume plants. Here we report the isolation and characterization of the D-genome copy of wheat CCaMK gene TaCCaMK. TaCCaMK was predominantly expressed in root tissues of wheat seedlings, and its proteins were located both on the cytoplasm membrane and in the nucleus as shown in the onion epidermis cells. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) assay showed that the expression of TaCCaMK was downregulated by ABA, as well as NaCl and PEG treatments in wheat seedling roots. A DNA fragment of 1,119 bp upstream of the start codon of the TaCCaMK gene (pTaCCaMK) was isolated by thermal asymmetric interlaced PCR (TAIL-PCR), on which six ABA-responsive cis-elements were predicted. pTaCCaMK can drive GUS reporter gene expression in the root and stem stalk of the transgenic Arabidopsis plants which can be repressed by ABA treatments, consistent with the observation in the qRT-PCR assay in wheat. Overexpressing TaCCaMK in Arabidopsis plants reduced their sensitivity to ABA treatment during seed germination and root elongation. Under high-salt conditions, the transgenic plants also conferred enhanced seed germination rate and became hypersensitive with increased chlorosis. Therefore, our data suggest that TaCCaMK is a negative regulator for ABA signaling which may participate in abiotic stress responses in wheat.

Ultrasensitive aptasensor with DNA tetrahedral nanostructure for Ochratoxin A detection based on hemin/G-quadruplex catalyzed polyaniline deposition

Abstract: An ultrasensitive electrochemical aptasensor with DNA tetrahedral nanostructure (DTN) for Ochratoxin A (OTA) detection was developed based on hemin/G-quadruplex catalyzed polyaniline (PANI) deposition in the presence of H2O2. DTN, which modified with OTA aptamer on top vertex and thiol group on other three vertices, was constructed on the modified electrode surface via Au-S bond and prominent template for PANI deposition owing to their numerous negative charges. The prepared gold nanorods (AuNRs) was used as a carrier to immobilize DTN and promote electron transfer. In the presence of OTA, the aptamer specific for OTA was formed G-quadruplex structure. The hemin/G-quadruplex was formed when hemin was intercalated into G-quadruplex, which had high catalytic activity to catalyze PANI deposition in the presence of H2O2 and amplify DPV signal. The amount of generated PANI was dependent on the number of hemin/G-quadruplex induced by OTA. Therefore, DPV currents was related to the concentration of OTA. The fabricated aptasensor exhibited a good linear response toward OTA over a wide range of concentrations from 0.001 ng/mL to 0.5 ng/mL, and the limit of detection of the proposed aptasensor was 0.26 pg/mL.

Sorption of perfluoroalkylated substances (PFASs) onto granular activated carbon and biochar.

Abstract: Perfluoroalkyl and polyfluoroalkyl substances (PFASs) have attracted increasing concerns owing to their potential ecotoxicological effects. The sorption of PFASs, i.e. perfluorooctanoic acid (PFOA)...