Jawaharlal Nehru University

About: Jawaharlal Nehru University is a education organization based out in New Delhi, India. It is known for research contribution in the topics: Population & Politics. The organization has 6082 authors who have published 13455 publications receiving 245407 citations. The organization is also known as: JNU.

Synthesis and Biological Evaluation of Novel Bisbenzimidazoles as Escherichia coli Topoisomerase IA Inhibitors and Potential Antibacterial Agents

Abstract: Novel bisbenzimidazole inhibitors of bacterial type IA topoisomerase are of interest for the development of new antibacterial agents that are impacted by target-mediated cross resistance with fluoroquinolones. The present study demonstrates the successful synthesis and evaluation of bisbenzimidazole analogues as Escherichia coli topoisomerase IA inhibitors. 5-(4-Propylpiperazin-1-yl)-2-[2′-(4-ethoxyphenyl)-5′-benzimidazolyl]benzimidazole (12b) showed significant relaxation inhibition activity against EcTopo 1A (IC50 = 2 ± 0.005 μM) and a tendency to chelate metal ion. Interestingly, these compounds did not show significant inhibition of E. coli DNA gyrase and hTop 1 even up to 100 μM. Compound 12b has shown lowest MIC against E. coli strains among 24 compounds evaluated. The binding affinity constant and binding free energy of 12b with EcTopo 1A was observed 6.8 × 106 M–1 and −10.84 kcal mol–1 from isothermal titration calorimetry (ITC), respectively. In vivo mouse systemic infection and neutropenic thigh...

Effect of Ionic Strength on Surface-Selective Patch Binding-Induced Phase Separation and Coacervation in Similarly Charged Gelatin−Agar Molecular Systems

Abstract: Coacervate is defined as a polymer-rich dense phase, which remains in thermodynamic equilibrium with its low concentrated phase called the supernatant. The effect of ionic strength (I = 0−0.1 M NaCl) on the mechanism of surface patch binding-induced protein−polysaccharide interaction leading to complex coacervation, between agar (a polyanionic polysaccharide) and gelatin B (a polyampholyte protein), both having similar net charge, at a particular mixing ratio, [gelatin]/[agar] = 1, was studied at various temperatures (20−40 °C). The coacervation transition was probed by turbidity and zeta-potential measurements. The intermolecular association had the signature of surface-selective binding, and a model calculation could explain the potential energy of interactions operative in such processes. The thermo-mechanical features of the coacervates were found to be strongly dependent on ionic strength, which has been interpreted as originating from formation of salt-bridges between the biopolymers. The microstruc...

Assessment of methane and nitrous oxide flux from mangroves along Eastern coast of India

Abstract: Mangroves are considered to be a minor source of greenhouse gases (CH4 and N2O) in pristine environmental condition. However, estimates of efflux suggest that anthropogenic activities have led to a pronounced increase in greenhouse gas emission. Along the east coast of India, mangroves vary substantially in area, physiography and freshwater input, which ultimately modify the biogeochemical processes operating within this ecosystem. An attempt has here been made to elucidate the existing variation and role of climatic variability on the emission of greenhouse gases from mangroves. The flux estimates of CH4 and N2O have been quantified from Bhitarkanika mangrove accounting for spatial and temporal (seasonal) variation. The annual rates were estimated to be 0.096 × 10 9 g CH4 year−1 and 5.8 × 103g N2O year−1 for the whole mangrove area of the east coast of India. Upscaling these estimates yield an annual emission of 1.95 × 10 12 g CH4 year−1 and 1.1 × 10 11 g N2O year−1 from worldwide mangrove areas. The influence of elevated nutrient inputs through anthropogenic influence enhances the emission of greenhouse gas. The present article shows the need to develop an inventory on greenhouse gas flux from mangrove ecosystem.