University of Massachusetts Amherst

About: University of Massachusetts Amherst is a education organization based out in Amherst Center, Massachusetts, United States. It is known for research contribution in the topics: Population & Galaxy. The organization has 37274 authors who have published 83965 publications receiving 3834996 citations. The organization is also known as: UMass Amherst & Massachusetts State College.

Apoptotic Signals Delivered Through the T-Cell Receptor of a T-Cell Hybrid Require the Immediate-Early Gene Nur77

Abstract: Engagement of the T-cell antigen receptor (TCR) on immature thymic T cells induces death by apoptosis. Although several lines of evidence indicate that apoptosis requires de novo gene expression, little is known about the molecular pathways that mediate this response. Here we show that nur77 (refs 4-7), a zinc-finger transcription factor, is expressed in response to TCR engagement in immature T cells and T-cell hybrids. Antisense inhibition of nur77 expression prevents apoptosis in TCR-stimulated cells. nur77 is also expressed in response to mitogens, but in this case transcription is regulated by 5' upstream elements that are distinct from those used for induction of apoptosis. In addition, polyadenylation is only observed on nur77 transcripts found in condemned cells. These data support a role for nur77 in cell death that may be distinct from that of activation.

Do all programmed cell deaths occur via apoptosis

Abstract: During development, large numbers of cells die by a nonpathological process referred to as programmed cell death. In many tissues, dying cells display similar changes in morphology and chromosomal DNA organization, which has been termed apoptosis. Apoptosis is such a widely documented phenomenon that many authors have assumed all programmed cell deaths occur by this process. Two well-characterized model systems for programmed cell death are (i) the death of T cells during negative selection in the mouse thymus and (ii) the loss of intersegmental muscles of the moth Manduca sexta at the end of metamorphosis. In this report we compare the patterns of cell death displayed by T cells and the intersegmental muscles and find that they differ in terms of cell-surface morphology, nuclear ultrastructure, DNA fragmentation, and polyubiquitin gene expression. Unlike the T cells, which are known to die via apoptosis, we find that the intersegmental muscles display few of the features that characterize apoptosis. These data suggest that more than one cell death mechanism is used during development.

An implementation of the contract net protocol based on marginal cost calculations

Abstract: This paper presents a formalization of the bidding and awarding decision process that was left undefined in the original contract net task allocation protocol This formalization is based on marginal cost calculations based on local agent criteria In this way, agents having very different local criteria (based on their selfinterest) can interact to distribute tasks so that the network as a whole functions more effectively In this model, both competitive and cooperative agents can interact In addition, the contract net protocol is extended to allow for clustering of tasks, to deal with the possibility of a large number of announcement and bid messages and to effectively handle situations, in which new bidding and awarding is being done during the period when the results of previous bids are unknown The protocol is verified by the TRACONET (TRAnsportation Cooperation' NET) system, where dispatch centers of different companies cooperate automatically in vehicle routing The implementation is asynchronous and truly distributed, and it provides the agents extensive autonomy The protocol is discussed in detail and test results with real data are presented