A consensus linkage map for molecular markers and Quantitative Trait Loci associated with economically important traits in melon (Cucumis melo L.)
Aurora Díaz,Mohamed Fergany,Mohamed Fergany,Gelsomina Formisano,Peio Ziarsolo,José Blanca,Zhanjun Fei,Jack E. Staub,Jack E. Staub,Juan Zalapa,Hugo E. Cuevas,Hugo E. Cuevas,Gayle Dace,M. Oliver,Nathalie Boissot,Catherine Dogimont,Michel Pitrat,René Hofstede,Paul van Koert,Rotem Harel-Beja,Galil Tzuri,Vitaly Portnoy,Shahar Cohen,Arthur A. Schaffer,Nurit Katzir,Yong Xu,Haiying Zhang,Nobuko Fukino,Satoru Matsumoto,Jordi Garcia-Mas,Antonio J. Monforte,Antonio J. Monforte +31 more
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TLDR
The integrated saturated map presented herein should be considered the initial reference map for melon and contains polymorphic in diverse collection of germplasm, and thus are potentially transferrable to a broad array of genetic experimentation.Abstract:
A number of molecular marker linkage maps have been developed for melon (Cucumis melo L.) over the last two decades. However, these maps were constructed using different marker sets, thus, making comparative analysis among maps difficult. In order to solve this problem, a consensus genetic map in melon was constructed using primarily highly transferable anchor markers that have broad potential use for mapping, synteny, and comparative quantitative trait loci (QTL) analysis, increasing breeding effectiveness and efficiency via marker-assisted selection (MAS). Under the framework of the International Cucurbit Genomics Initiative (ICuGI, http://www.icugi.org
), an integrated genetic map has been constructed by merging data from eight independent mapping experiments using a genetically diverse array of parental lines. The consensus map spans 1150 cM across the 12 melon linkage groups and is composed of 1592 markers (640 SSRs, 330 SNPs, 252 AFLPs, 239 RFLPs, 89 RAPDs, 15 IMAs, 16 indels and 11 morphological traits) with a mean marker density of 0.72 cM/marker. One hundred and ninety-six of these markers (157 SSRs, 32 SNPs, 6 indels and 1 RAPD) were newly developed, mapped or provided by industry representatives as released markers, including 27 SNPs and 5 indels from genes involved in the organic acid metabolism and transport, and 58 EST-SSRs. Additionally, 85 of 822 SSR markers contributed by Syngenta Seeds were included in the integrated map. In addition, 370 QTL controlling 62 traits from 18 previously reported mapping experiments using genetically diverse parental genotypes were also integrated into the consensus map. Some QTL associated with economically important traits detected in separate studies mapped to similar genomic positions. For example, independently identified QTL controlling fruit shape were mapped on similar genomic positions, suggesting that such QTL are possibly responsible for the phenotypic variability observed for this trait in a broad array of melon germplasm. Even though relatively unsaturated genetic maps in a diverse set of melon market types have been published, the integrated saturated map presented herein should be considered the initial reference map for melon. Most of the mapped markers contained in the reference map are polymorphic in diverse collection of germplasm, and thus are potentially transferrable to a broad array of genetic experimentation (e.g., integration of physical and genetic maps, colinearity analysis, map-based gene cloning, epistasis dissection, and marker-assisted selection).read more
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The genome of melon (Cucumis melo L.)
Jordi Garcia-Mas,Andrej Benjak,Walter Sanseverino,Michael Bourgeois,Gisela Mir,Víctor M. González,Elizabeth Henaff,Francisco Câmara,Luca Cozzuto,Ernesto Lowy,Tyler Alioto,Salvador Capella-Gutierrez,José Blanca,Joaquín Cañizares,Pello Ziarsolo,Daniel Gonzalez-Ibeas,Luis Rodriguez-Moreno,Marcus Droege,Lei Du,Miguel Álvarez-Tejado,Belen Lorente-Galdos,Marta Melé,Marta Melé,Luming Yang,Yiqun Weng,Arcadi Navarro,Tomas Marques-Bonet,Miguel A. Aranda,Fernando Nuez,Belén Picó,Toni Gabaldón,Guglielmo Roma,Roderic Guigó,Josep M. Casacuberta,Pere Arús,Pere Puigdomènech +35 more
TL;DR: The data suggest that transposon amplification may in part explain the increased size of the melon genome compared with the close relative cucumber, and a low number of nucleotide-binding site–leucine-rich repeat disease resistance genes were annotated, suggesting the existence of specific defense mechanisms in this species.
Journal ArticleDOI
The genetic basis of fruit morphology in horticultural crops: lessons from tomato and melon
TL;DR: It is found that QTLs for fruit weight co-localized frequently with members of the CNR/FW2.2 and KLUH/FW3.2 families, as well as co- localizations between OFP family members and fruit-shape Q TLs, making this family the most suitable to explain fruit shape variation among melon accessions.
Journal ArticleDOI
A new genomic library of melon introgression lines in a cantaloupe genetic background for dissecting desirable agronomical traits
TL;DR: A new melon IL collection in the Charentais genetic background has been developed and genomic regions that consistently affect flowering and fruit quality traits have been identified, which demonstrates the suitability of this collection for dissecting complex traits in melon.
Journal ArticleDOI
Variability of candidate genes, genetic structure and association with sugar accumulation and climacteric behavior in a broad germplasm collection of melon ( Cucumis melo L.)
Carmen Alice Leida,C. Moser,Cristina Esteras,Ronan Sulpice,Ronan Sulpice,John E. Lunn,Frank de Langen,Antonio J. Monforte,Belén Picó +8 more
TL;DR: An important variability at both the phenotypic and candidate gene levels for ripening behavior and sugar accumulation in melon fruit is found and several candidate genes that may be involved in the melon phenotypesic diversity are identified.
Journal ArticleDOI
A ‘golden’ SNP in CmOr governs the fruit flesh color of melon (Cucumis melo)
Galil Tzuri,Xiangjun Zhou,Noam Chayut,Noam Chayut,Hui Yuan,Vitaly Portnoy,Ayala Meir,Uzi Saar,Fabian Baumkoler,Michael Mazourek,Efraim Lewinsohn,Zhangjun Fei,Arthur A. Schaffer,Li Li,Joseph Burger,Nurit Katzir,Yaakov Tadmor +16 more
TL;DR: The identification of the 'golden' SNP in CmOr, which is responsible for the non-orange and orange melon fruit phenotypes, provides new tools for studying the Or mechanism of action, and suggests genome editing of the Or gene for nutritional biofortification of crops.
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TL;DR: This study establishes that five of the cucumber's seven chromosomes arose from fusions of ten ancestral chromosomes after divergence from Cucumis melo, and identifies 686 gene clusters related to phloem function.
Journal ArticleDOI
Genetic and molecular regulation of fruit and plant domestication traits in tomato and pepper
Ilan Paran,Esther van der Knaap +1 more
TL;DR: This review will present an overview of the history of tomato and pepper and discuss key plant traits that were specifically selected during domestication of the two species, questioning whether mutations at orthologous loci occurred independently in these two species or whether unique plant and fruit features resulted in selection at different genes.
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