A multi-step transcriptional and chromatin state cascade underlies motor neuron programming from embryonic stem cells
Silvia Velasco,Mahmoud M. Ibrahim,Mahmoud M. Ibrahim,Akshay Kakumanu,Görkem Garipler,Begüm Aydin,Mohamed Al-Sayegh,Mohamed Al-Sayegh,Antje Hirsekorn,Farah Abdul-Rahman,Rahul Satija,Uwe Ohler,Uwe Ohler,Shaun Mahony,Esteban O. Mazzoni +14 more
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TLDR
This analysis reveals a highly dynamic process in which Ngn2 and the Isl1/Lhx3 pair initially engage distinct regulatory regions, and motor neuron programming is the product of two initially independent transcriptional modules that converge with a feedforward transcriptional logic.About:
This article is published in Cell Stem Cell.The article was published on 2017-02-02 and is currently open access. It has received 84 citations till now. The article focuses on the topics: Cellular differentiation & Chromatin.read more
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Smart-seq2 for sensitive full-length transcriptome profiling in single cells
Simone Picelli,Åsa K. Björklund,Åsa K. Björklund,Omid R. Faridani,Sven Sagasser,Sven Sagasser,Gösta Winberg,Gösta Winberg,Rickard Sandberg,Rickard Sandberg +9 more
TL;DR: Smart-seq2 as discussed by the authors improved reverse transcription, template switching and preamplification to increase both yield and length of cDNA libraries generated from individual cells, which have improved detection, coverage, bias and accuracy compared to Smart-seq libraries and are generated with off-the-shelf reagents at lower cost.
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Establishing neuronal diversity in the spinal cord: a time and a place
Andreas Sagner,James Briscoe +1 more
TL;DR: This Review outlines how distinct neuronal cell identities are established in response to spatial and temporal patterning systems, and outlines novel experimental approaches to study the emergence and function of neuronal diversity in the spinal cord.
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Cortical interneuron development: a tale of time and space.
TL;DR: New insights into cortical GABAergic interneuron subtype specification are examined, focussing on spatial, temporal and genetic mechanisms regulating cell fate decisions in the mouse medial ganglionic eminence.
Journal ArticleDOI
Rapid Chromatin Switch in the Direct Reprogramming of Fibroblasts to Neurons
Orly L. Wapinski,Qian Yi Lee,A.C. Chen,Rui Li,M. Ryan Corces,Cheen Euong Ang,Barbara Treutlein,Barbara Treutlein,Chaomei Xiang,Chaomei Xiang,Valerie Baubet,Valerie Baubet,Fabian P. Suchy,Venkat Sankar,Sopheak Sim,Stephen R. Quake,Nadia Dahmane,Nadia Dahmane,Marius Wernig,Howard Y. Chang +19 more
TL;DR: In this article, the authors defined chromatin accessibility changes induced by the proneural transcription factor Ascl1 throughout conversion of fibroblasts into induced neuronal (iN) cells, and found that over 80% of the accessibility changes occur between days 2 and 5 of the 3-week reprogramming process.
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Neural stem cell differentiation into mature neurons: Mechanisms of regulation and biotechnological applications
Mariana S. Vieira,Anderson Kury Santos,Rebecca Vasconcellos,Vânia Aparecida Mendes Goulart,Ricardo Parreira,Alexandre Hiroaki Kihara,Henning Ulrich,Rodrigo R. Resende +7 more
TL;DR: A better understanding of the mechanisms underlying how the process of neurogenesis is induced, regulated, and maintained will provide elues for development of novel for strategies for neurodegenerative therapies.
References
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Heng Li,Bob Handsaker,Alec Wysoker,T. J. Fennell,Jue Ruan,Nils Homer,Gabor T. Marth,Gonçalo R. Abecasis,Richard Durbin +8 more
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