Bone Tissue Engineering: Recent Advances and Challenges
TLDR
The fundamentals of bone tissue engineering are discussed, highlighting the current state of this field, and the recent advances of biomaterial and cell-based research, as well as approaches used to enhance bone regeneration.Abstract:
The worldwide incidence of bone disorders and conditions has trended steeply upward and is expected to double by 2020, especially in populations where aging is coupled with increased obesity and poor physical activity. Engineered bone tissue has been viewed as a potential alternative to the conventional use of bone grafts, due to their limitless supply and no disease transmission. However, bone tissue engineering practices have not proceeded to clinical practice due to several limitations or challenges. Bone tissue engineering aims to induce new functional bone regeneration via the synergistic combination of biomaterials, cells, and factor therapy. In this review, we discuss the fundamentals of bone tissue engineering, highlighting the current state of this field. Further, we review the recent advances of biomaterial and cell-based research, as well as approaches used to enhance bone regeneration. Specifically, we discuss widely investigated biomaterial scaffolds, micro- and nano-structural properties of these scaffolds, and the incorporation of biomimetic properties and/or growth factors. In addition, we examine various cellular approaches, including the use of mesenchymal stem cells (MSCs), embryonic stem cells (ESCs), adult stem cells, induced pluripotent stem cells (iPSCs), and platelet-rich plasma (PRP), and their clinical application strengths and limitations. We conclude by overviewing the challenges that face the bone tissue engineering field, such as the lack of sufficient vascularization at the defect site, and the research aimed at functional bone tissue engineering. These challenges will drive future research in the field.read more
Citations
More filters
Journal ArticleDOI
A 3D bioprinting system to produce human-scale tissue constructs with structural integrity
TL;DR: An integrated tissue–organ printer (ITOP) that can fabricate stable, human-scale tissue constructs of any shape is presented and the incorporation of microchannels into the tissue constructs facilitates diffusion of nutrients to printed cells, thereby overcoming the diffusion limit of 100–200 μm for cell survival in engineered tissues.
Journal ArticleDOI
An Overview of Poly(lactic-co-glycolic) Acid (PLGA)-Based Biomaterials for Bone Tissue Engineering
TL;DR: The analysis of the state of the art in the field reveals the presence of current innovative techniques for scaffolds and material manufacturing that are currently opening the way to prepare biomimetic PLGA substrates able to modulate cell interaction for improved substitution, restoration, or enhancement of bone tissue function.
Journal ArticleDOI
Scaffolds for Bone Tissue Engineering: State of the art and new perspectives.
Livia Roseti,V. Parisi,Mauro Petretta,Carola Cavallo,Giovanna Desando,Isabella Bartolotti,Brunella Grigolo +6 more
TL;DR: It is highlighted that, despite its encouraging results, the clinical approach of Bone Tissue Engineering has not taken place on a large scale yet, due to the need of more in depth studies, its high manufacturing costs and the difficulty to obtain regulatory approval.
Journal ArticleDOI
3D bioactive composite scaffolds for bone tissue engineering.
Gareth Turnbull,Jon Clarke,Frederic Picard,Frederic Picard,Philip Riches,Luanluan Jia,Fengxuan Han,Bin Li,Wenmiao Shu +8 more
TL;DR: This review will consider the ideal properties of bioactive composite 3D scaffolds and examine recent use of polymers, hydrogels, metals, ceramics and bio-glasses in BTE.
Journal ArticleDOI
Scaffold Design for Bone Regeneration
TL;DR: This focus of this review is on the evolution of these scaffolds as bone graft substitutes in the process of recreating the bone tissue microenvironment, including biochemical and biophysical cues.
References
More filters
Journal ArticleDOI
Thrombin peptide (TP508) promotes fracture repair by up-regulating inflammatory mediators, early growth factors, and increasing angiogenesis
Hali Wang,Xin-Min Li,Emre Tomin,Stephen B. Doty,Joseph M. Lane,Darrell H. Carney,James T. Ryaby +6 more
TL;DR: It is suggested that TP508 promotes fracture repair through a mechanism that involves an increased induction of a number of growth factors, enhanced expression of inflammatory mediators, and angiogenesis‐related genes.
Journal ArticleDOI
In vitro study of collagen coating of titanium implants for initial cell attachment.
Megumi Nagai,Tohru Hayakawa,Akira Fukatsu,Masafumi Yamamoto,Masahiko Fukumoto,Fumio Nagahama,Mishima H,Masao Yoshinari,Kimiya Nemoto,Takao Kato +9 more
TL;DR: It is expected that collagen coating of titanium implants will improve the attachment of the peri-implant soft tissue to titanium at early stages after the implantation.
Journal ArticleDOI
Safety of autologous bone marrow aspiration concentrate transplantation: initial experiences in 101 patients
TL;DR: The on-site preparation of the bone marrow cells within the operating theater eliminates the specific risk of ex vivo cell proliferation and has a safety advantage in the use of autologous cell therapy for bone regeneration.
Journal ArticleDOI
Methods for the Identification, Characterization and Banking of Human DPSCs: Current Strategies and Perspectives
Virginia Tirino,Francesca Paino,Riccardo d'Aquino,Vincenzo Desiderio,Alfredo De Rosa,Gianpaolo Papaccio +5 more
TL;DR: This report focuses on the main procedures used in the selection of DPSCs by flow cytometry, cell culture, freezing/thawing, cell cycle evaluation, histochemistry/immunofluorescence and differentiation of DPSC selection.
Journal ArticleDOI
Synergistic induction of bone formation by hOP-1, hTGF-β3 and inhibition by zoledronate in macroporous coral-derived hydroxyapatites
TL;DR: The lack ofBone formation by zoledronate-treated specimens indicates that osteoclastic activity on the implanted coral-derived constructs is critical for the spontaneous induction of bone formation, not comparable however to synergistic binary applications.