Journal ArticleDOI
Chemical modification of chitosan as a gene carrier in vitro and in vivo
Tae Hee Kim,Hu-Lin Jiang,Dhananjay Jere,In-Kyu Park,Myung-Haing Cho,Jae-Woon Nah,Yun-Jaie Choi,Toshihiro Akaike,Chong-Su Cho +8 more
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TLDR
The role of chitosan as a carrier of controlled release of DNA and small interfering RNA is described and the role of several factors for the enhancement of transfection efficiency and cell specificity in vitro are summarized.About:
This article is published in Progress in Polymer Science.The article was published on 2007-07-01. It has received 304 citations till now. The article focuses on the topics: Chitosan & Gene delivery.read more
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Nonviral Vectors for Gene Delivery
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
Journal ArticleDOI
Chitosan-based biomaterials for tissue engineering
TL;DR: The preparation and properties of innovative chitosan-based biomaterials, with respect to their future applications, are highlighted, with a special focus on wound healing application.
Journal ArticleDOI
Polysaccharides-based nanoparticles as drug delivery systems
TL;DR: In this review, four mechanisms are introduced to prepare polysaccharides-based nanoparticles, that is, covalent crosslinking, ionic crossl linking, polyelectrolyte complex, and the self-assembly of hydrophobically modified poly Saccharides.
Journal ArticleDOI
Chitosan-based formulations for delivery of DNA and siRNA.
Shirui Mao,Wei Sun,Thomas Kissel +2 more
TL;DR: Chitosan structure modification or additive incorporation is an effective way to improve the stability of the polyplex in biological fluids, enhance targeted cell delivery and facilitate endo-lysosomal release of the complex.
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Current research on the blends of natural and synthetic polymers as new biomaterials: Review
TL;DR: In this article, the structure, preparation and properties of the blends of natural and man-made polymers are discussed in general, and detailed examples are also drawn from scientific literature and practical work.
References
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Journal ArticleDOI
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Andrew Fire,SiQun Xu,Mary K. Montgomery,Steven A. Kostas,Steven A. Kostas,Samuel E. Driver,Craig C. Mello +6 more
TL;DR: To their surprise, it was found that double-stranded RNA was substantially more effective at producing interference than was either strand individually, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.
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Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
TL;DR: 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
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A versatile vector for gene and oligonucleotide transfer into cells in culture and in vivo: polyethylenimine
Otmane Boussif,Frank Lezoualc'h,Maria Antonietta Zanta,Mojgan Mergny,Daniel Scherman,Barbara A. Demeneix,Jean-Paul Behr +6 more
TL;DR: Together, these properties make PEI a promising vector for gene therapy and an outstanding core for the design of more sophisticated devices because its efficiency relies on extensive lysosome buffering that protects DNA from nuclease degradation, and consequent lysOSomal swelling and rupture that provide an escape mechanism for the PEI/DNA particles.
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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
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Role for a bidentate ribonuclease in the initiation step of RNA interference
TL;DR: Dicer is a member of the RNase III family of nucleases that specifically cleave double-stranded RNAs, and is evolutionarily conserved in worms, flies, plants, fungi and mammals, and has a distinctive structure, which includes a helicase domain and dualRNase III motifs.