Clonal integration of a polyomavirus in human Merkel cell carcinoma.
TLDR
In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells, and MCV may be a contributing factor in the pathogenesis of MCC.Abstract:
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction and detected a fusion transcript between a previously undescribed virus T antigen and a human receptor tyrosine phosphatase. Further investigation led to identification and sequence analysis of the 5387-base-pair genome of a previously unknown polyomavirus that we call Merkel cell polyomavirus (MCV or MCPyV). MCV sequences were detected in 8 of 10 (80%) MCC tumors but only 5 of 59 (8%) control tissues from various body sites and 4 of 25 (16%) control skin tissues. In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells. Thus, MCV may be a contributing factor in the pathogenesis of MCC.read more
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Detection and characterization of a novel polyomavirus in wild rodents.
Yasuko Orba,Shintaro Kobayashi,Ichiro Nakamura,Akihiro Ishii,Bernard M. Hang’ombe,Aaron S. Mweene,Yuka Thomas,Takashi Kimura,Hirofumi Sawa +8 more
TL;DR: Comparison of large T antigen function in mastomys polyomvirus with that in rhesus monkey polyomavirus SV40 and human polyomAVirus JC virus revealed that the large T antibody interacted with the tumour suppressor protein pRb, but not with p53.
Journal ArticleDOI
Bcl-2 expression indicates better prognosis of Merkel cell carcinoma regardless of the presence of Merkel cell polyomavirus
TL;DR: Bcl-2 positivity indicates better clinical stage at the time of diagnosis and a longer survival in MCC, and the recently found Merkel cell polyomavirus integrates clonally in the tumour genome, which suggests an important role in the pathogenesis of the disease.
Journal ArticleDOI
Tumor-infiltrating lymphocytes and outcome in Merkel cell carcinoma, a virus-associated cancer.
Harri Sihto,Heikki Joensuu +1 more
TL;DR: High tumor-infiltrating T-cell counts are associated with favorable survival regardless of the tumor MCPyV status, so boosting host immune functions might constitute a new approach for the treatment of MCC.
Journal ArticleDOI
Genomics of High-Grade Neuroendocrine Neoplasms: Well-Differentiated Neuroendocrine Tumor with High-Grade Features (G3 NET) and Neuroendocrine Carcinomas (NEC) of Various Anatomic Sites.
Silvia Uccella,Stefano La Rosa,Jasna Metovic,Deborah Marchiori,Jean-Yves Scoazec,Marco Volante,Ozgur Mete,Ozgur Mete,Mauro Papotti +8 more
TL;DR: In this paper, a review systematically addresses the genomic landscape of high grade neuroendocrine neoplasm throughout the more common thoracic and digestive locations, as well as it will consider other rare but not exceptional primary sites, including the skin, the head and neck, and the urogenital system.
Journal ArticleDOI
Complete Spontaneous Regression of Merkel Cell Carcinoma (1986–2016): A 30 year perspective
TL;DR: This case served as the prototype for examples of CSR of MCC to follow, and invoked cellular immunity as the likely mechanism of regression, although their own microscopic evaluation had not disclosed evidence to support this hypothesis.
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