Clonal integration of a polyomavirus in human Merkel cell carcinoma.
TLDR
In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells, and MCV may be a contributing factor in the pathogenesis of MCC.Abstract:
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction and detected a fusion transcript between a previously undescribed virus T antigen and a human receptor tyrosine phosphatase. Further investigation led to identification and sequence analysis of the 5387-base-pair genome of a previously unknown polyomavirus that we call Merkel cell polyomavirus (MCV or MCPyV). MCV sequences were detected in 8 of 10 (80%) MCC tumors but only 5 of 59 (8%) control tissues from various body sites and 4 of 25 (16%) control skin tissues. In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells. Thus, MCV may be a contributing factor in the pathogenesis of MCC.read more
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Human polyomaviruses 6 and 7 are not detectable in Merkel cell polyomavirus-negative Merkel cell carcinoma.
TL;DR: No reliable microscopic, clinical, phenotypic or molecular differences have been identified that distinguish MCPyV‐positive from MCPYV‐negative MCC, raising the possibility that a related polyomavirus may be present in MCPi‐negative cases.
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Distinction of 2 different primary Merkel cell carcinomas in 1 patient by Merkel cell polyomavirus genome analysis.
David Schrama,Anja Thiemann,Roland Houben,Katharina C. Kähler,Jürgen C. Becker,Axel Hauschild +5 more
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Activities of Different Classes of Acyclic Nucleoside Phosphonates against BK Virus in Primary Human Renal Cells
TL;DR: Evaluation of three different classes of acyclic nucleoside phosphonates for their activities against BKV replication in two different primary renal cells: renal proximal tubular epithelial cells (RPTECs) and human renal cortical epithelial (HRCE) cells indicates that besides HPMPC and its cyclic form, (S)-1-[3-hydroxy-2-(phosphonomethoxy)propyl]-5-azacytosine
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Novel approaches for managing aged skin and nonmelanoma skin cancer.
TL;DR: The most recent findings in skin aging and nonmelanoma skin cancer are reviewed, including addition to traditional and developing therapies.
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Merkel cell polyomavirus and non-Merkel cell carcinomas: guilty or circumstantial evidence?
TL;DR: The current review is focusing on the available data describing the presence of MCPyV in non‐MCC tumors, with an aim to provide a comprehensive overview of the corresponding literature and to discuss the potential contribution of M CPyV to non-MCC cancer in light of this.
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