Clonal integration of a polyomavirus in human Merkel cell carcinoma.
TLDR
In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells, and MCV may be a contributing factor in the pathogenesis of MCC.Abstract:
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction and detected a fusion transcript between a previously undescribed virus T antigen and a human receptor tyrosine phosphatase. Further investigation led to identification and sequence analysis of the 5387-base-pair genome of a previously unknown polyomavirus that we call Merkel cell polyomavirus (MCV or MCPyV). MCV sequences were detected in 8 of 10 (80%) MCC tumors but only 5 of 59 (8%) control tissues from various body sites and 4 of 25 (16%) control skin tissues. In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells. Thus, MCV may be a contributing factor in the pathogenesis of MCC.read more
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Seroprevalence of Merkel cell polyomavirus in the general rural population of Anyang, China.
Chanyuan Zhang,Fangfang Liu,Zhonghu He,Qiuju Deng,Yaqi Pan,Ying Liu,Chaoting Zhang,Tao Ning,Chuanhai Guo,Yongmei Liang,Ruiping Xu,Lixin Zhang,Hong Cai,Yang Ke +13 more
TL;DR: Serological evidence suggests that nonsexual horizontal spread of MCPyV can occur among family members, and further research in this regard is needed.
Journal ArticleDOI
Sentinel lymph node biopsy in patients with Merkel cell carcinoma: An emerging role and the Westmead hospital experience
Julie Howle,Michael J. Veness +1 more
TL;DR: This work sought to calculate the rates of positive and false negative SLNB and to evaluate the impact of SLNB on the staging and management of patients with MCC at this institution.
Journal ArticleDOI
S2k guidelines for Merkel cell carcinoma (MCC, neuroendocrine carcinoma of the skin) – update 2018
Jürgen C. Becker,Thomas Eigentler,Bernhard Frerich,Thilo Gambichler,Stephan Grabbe,Ulrike Höller,Bernhard Klumpp,Carmen Loquai,Albrecht Krause-Bergmann,Urs Müller-Richter,Claudia Pföhler,Sylke Schneider-Burrus,Andreas Stang,Patrick Terheyden,Selma Ugurel,Johannes Veith,Cornelia Mauch +16 more
TL;DR: Given the frequent occurrence of occult lymph node metastasis, sentinel lymph node biopsy should be performed once distant metastasis has been ruled out by cross‐sectional imaging.
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Culture-Independent Diagnostics for Health Security.
Norman A. Doggett,Harshini Mukundan,Elliot J. Lefkowitz,Tom Slezak,Patrick Sam Chain,Stephen Morse,Kevin Anderson,David R. Hodge,Segaran P. Pillai +8 more
TL;DR: The key features, benefits, and limitations of the various CIDT methods for providing rapid pathogen identification that are critical to the response and mitigation of a bioterrorism event are identified.
Journal ArticleDOI
Age and Gender Associations of Virus Positivity in Merkel Cell Carcinoma Characterized Using a Novel RNA In Situ Hybridization Assay
Lisha Wang,Paul W. Harms,Nallasivam Palanisamy,Nallasivam Palanisamy,Shannon Carskadon,Xuhong Cao,Xuhong Cao,Javed Siddiqui,Rajiv M. Patel,Sylvia Zelenka-Wang,Alison B. Durham,Douglas R. Fullen,Kelly L. Harms,Fengyun Su,Sudhanshu Shukla,Rohit Mehra,Arul M. Chinnaiyan +16 more
TL;DR: RNA-ISH is comparably sensitive to qPCR for detection of MCPyV and allows for correlation with tissue morphology, and this study reveals a significant association between age, gender, and M CPyV positivity.
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