Clonal integration of a polyomavirus in human Merkel cell carcinoma.
TLDR
In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells, and MCV may be a contributing factor in the pathogenesis of MCC.Abstract:
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction and detected a fusion transcript between a previously undescribed virus T antigen and a human receptor tyrosine phosphatase. Further investigation led to identification and sequence analysis of the 5387-base-pair genome of a previously unknown polyomavirus that we call Merkel cell polyomavirus (MCV or MCPyV). MCV sequences were detected in 8 of 10 (80%) MCC tumors but only 5 of 59 (8%) control tissues from various body sites and 4 of 25 (16%) control skin tissues. In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells. Thus, MCV may be a contributing factor in the pathogenesis of MCC.read more
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Excretion of the novel polyomaviruses KI and WU in the stool of patients with hematological disorders.
Muhammed Babakir-Mina,Massimo Ciccozzi,Claudia Alteri,Paola Polchi,Alessandra Picardi,Francesco Greco,Guido Lucarelli,William Arcese,Carlo Federico Perno,Marco Ciotti +9 more
TL;DR: Stool specimens from 31 patients with hematological disorders were examined prospectively to determine whether the novel polyomaviruses KIV and WUV reactivated and were excreted in the gastrointestinal tract andylogenetic analysis of the small t‐antigen gene showed that the novels identified in feces clustered with those identified in the respiratory tract suggesting an oral–fecal transmission of these viruses.
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Developing a sense of touch.
Blair A Jenkins,Ellen A. Lumpkin +1 more
TL;DR: This Review presents current knowledge of the development of the sensory microdomains in mammalian skin and the mechanosensory neurons that innervate them.
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The Merkel cell polyomavirus and its involvement in Merkel cell carcinoma.
TL;DR: The host's immune function appears to affect MCV's ability to cause cellular transformation leading to MCC, and an understanding of this virus may aid in future therapy options and current pathology protocols in diagnosing MCC.
Journal ArticleDOI
Translational research in infectious disease: current paradigms and challenges ahead
TL;DR: This article will review, in a noninclusive fashion, several recent scientific advancements in the field of translational research, with a specific focus on how they relate to infectious disease.
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