Clonal integration of a polyomavirus in human Merkel cell carcinoma.
TLDR
In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells, and MCV may be a contributing factor in the pathogenesis of MCC.Abstract:
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction and detected a fusion transcript between a previously undescribed virus T antigen and a human receptor tyrosine phosphatase. Further investigation led to identification and sequence analysis of the 5387-base-pair genome of a previously unknown polyomavirus that we call Merkel cell polyomavirus (MCV or MCPyV). MCV sequences were detected in 8 of 10 (80%) MCC tumors but only 5 of 59 (8%) control tissues from various body sites and 4 of 25 (16%) control skin tissues. In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells. Thus, MCV may be a contributing factor in the pathogenesis of MCC.read more
Citations
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Discovery of functional genomic motifs in viruses with ViReMa-a Virus Recombination Mapper-for analysis of next-generation sequencing data
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The trichodysplasia spinulosa-associated polyomavirus: virological background and clinical implications
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Merkel cell carcinoma - a population-based epidemiological study in Finland with a clinical series of 181 cases.
Heli Kukko,Tom Böhling,Virve Koljonen,Erkki Tukiainen,Caj Haglund,Arun Pokhrel,Risto Sankila,Eero Pukkala +7 more
TL;DR: The results support the view that complete excision with clear margins and post operative radiotherapy decrease local recurrences in Merkel cell carcinoma patients in Finland, with incidence rates similar to those in the other Nordic countries.
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Evolution and Emergence of Pathogenic Viruses: Past, Present, and Future.
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Metagenomic identification of viral pathogens
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