Clonal integration of a polyomavirus in human Merkel cell carcinoma.
TLDR
In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells, and MCV may be a contributing factor in the pathogenesis of MCC.Abstract:
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction and detected a fusion transcript between a previously undescribed virus T antigen and a human receptor tyrosine phosphatase. Further investigation led to identification and sequence analysis of the 5387-base-pair genome of a previously unknown polyomavirus that we call Merkel cell polyomavirus (MCV or MCPyV). MCV sequences were detected in 8 of 10 (80%) MCC tumors but only 5 of 59 (8%) control tissues from various body sites and 4 of 25 (16%) control skin tissues. In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells. Thus, MCV may be a contributing factor in the pathogenesis of MCC.read more
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Review on the role of the human Polyomavirus JC in the development of tumors
TL;DR: The published literature is summarized and critically analyzed, in order to describe the current knowledge on the possible role of JCPyV in the development of human tumors.
Journal ArticleDOI
Use of Illumina Deep Sequencing Technology To Differentiate Hepatitis C Virus Variants
Masashi Ninomiya,Yoshiyuki Ueno,Ryo Funayama,Takeshi Nagashima,Yuichiro Nishida,Yasuteru Kondo,Jun Inoue,Eiji Kakazu,Osamu Kimura,Keiko Nakayama,Tooru Shimosegawa +10 more
TL;DR: The successful use of Illumina deep sequencing technology and subsequent analyses to determine the genetic variants and amino acid substitutions in both treatment-naïve and treatment-experienced isolates from HCV-infected patients are demonstrated.
Journal ArticleDOI
The Genomics of Emerging Pathogens
Cadhla Firth,W. Ian Lipkin +1 more
TL;DR: Advances in methods for microbial discovery and characterization, as well as strategies for testing the clinical and public health significance of microbe-disease associations are reviewed, and how genetic data can inform the general process of pathogen emergence is discussed.
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Inhibition of Simian Virus 40 replication by targeting the molecular chaperone function and ATPase activity of T antigen.
Christine M. Wright,Sandlin P. Seguin,Sheara W. Fewell,Haijiang Zhang,Chandra Ishwad,Abhay Vats,Clifford A. Lingwood,Peter Wipf,Ellen Fanning,James M. Pipas,Jeffrey L. Brodsky +10 more
TL;DR: Data indicate that pharmacological inhibition of TAg's chaperone and ATPase activities may provide a route to combat polyomavirus-mediated disease.
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Human polyomaviruses in children undergoing transplantation, United States, 2008-2010.
Erica A. Siebrasse,Irma K. Bauer,Lori R. Holtz,Binh-Minh N Le,Sherry Lassa-Claxton,Charles E. Canter,Paul Hmiel,Shalini Shenoy,Stuart C. Sweet,Yumirle P. Turmelle,Ross W. Shepherd,David Wang +11 more
TL;DR: Samples from a longitudinal cohort were collected and the prevalence of polyomaviruses in children undergoing transplantation was defined, finding several were present in previously unreported specimen types.
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