Clonal integration of a polyomavirus in human Merkel cell carcinoma.
TLDR
In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells, and MCV may be a contributing factor in the pathogenesis of MCC.Abstract:
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction and detected a fusion transcript between a previously undescribed virus T antigen and a human receptor tyrosine phosphatase. Further investigation led to identification and sequence analysis of the 5387-base-pair genome of a previously unknown polyomavirus that we call Merkel cell polyomavirus (MCV or MCPyV). MCV sequences were detected in 8 of 10 (80%) MCC tumors but only 5 of 59 (8%) control tissues from various body sites and 4 of 25 (16%) control skin tissues. In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells. Thus, MCV may be a contributing factor in the pathogenesis of MCC.read more
Citations
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Merkel cell polyomavirus DNA in respiratory specimens from children and adults
TL;DR: Partial T antigen and major capsid protein sequences of MCPyV identified in respiratory secretions showed high homology to those found in Merkel cell carcinoma.
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Genetic variability and integration of Merkel cell polyomavirus in Merkel cell carcinoma.
Claire Martel-Jantin,Claudia Filippone,Olivier Cassar,Martine Peter,Gorana Tomasic,Philippe Vielh,Josette Brière,Tony Petrella,Marie-Hélène Aubriot-Lorton,Laurent Mortier,Grégory Jouvion,Xavier Sastre-Garau,Caroline Robert,Antoine Gessain +13 more
TL;DR: MCPyV integration was demonstrated in 19 out of 27 FF MCC DNA biopsies without evidence of specific host cellular genome integration site, and the viral junction was located within the second exon of the LTAg, after the pRB binding domain.
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The rapidly expanding family of human polyomaviruses: recent developments in understanding their life cycle and role in human pathology.
TL;DR: The problems associated with studying HPyVs, such as the lack of culture systems for the new viruses and the gaps in basic understanding of their biology are discussed, are discussed.
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Serologic evidence of frequent human infection with WU and KI polyomaviruses
TL;DR: WU and KI polyomavirus infections are widespread and need to be addressed.
Journal ArticleDOI
Epigenetic priming restores the HLA class-I antigen processing machinery expression in Merkel cell carcinoma.
Cathrin Ritter,Cathrin Ritter,Kaiji Fan,Kaiji Fan,Annette Paschen,Sine Reker Hardrup,Soldano Ferrone,Paul Nghiem,Selma Ugurel,David Schrama,Jürgen C. Becker +10 more
TL;DR: Restoration of HLA class-I expression on MCC cells by epigenetic priming is an attractive approach to enhance therapies boosting adaptive immune responses.
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