Clonal integration of a polyomavirus in human Merkel cell carcinoma.
TLDR
In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells, and MCV may be a contributing factor in the pathogenesis of MCC.Abstract:
Merkel cell carcinoma (MCC) is a rare but aggressive human skin cancer that typically affects elderly and immunosuppressed individuals, a feature suggestive of an infectious origin. We studied MCC samples by digital transcriptome subtraction and detected a fusion transcript between a previously undescribed virus T antigen and a human receptor tyrosine phosphatase. Further investigation led to identification and sequence analysis of the 5387-base-pair genome of a previously unknown polyomavirus that we call Merkel cell polyomavirus (MCV or MCPyV). MCV sequences were detected in 8 of 10 (80%) MCC tumors but only 5 of 59 (8%) control tissues from various body sites and 4 of 25 (16%) control skin tissues. In six of eight MCV-positive MCCs, viral DNA was integrated within the tumor genome in a clonal pattern, suggesting that MCV infection and integration preceded clonal expansion of the tumor cells. Thus, MCV may be a contributing factor in the pathogenesis of MCC.read more
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An Update on Tumors of the Lacrimal Gland.
Simon Andreasen,Bita Esmaeli,Sarah Linéa von Holstein,Lauge Hjorth Mikkelsen,Peter Kristian Rasmussen,Steffen Heegaard +5 more
TL;DR: An update on the clinical, radiological, histological, and molecular features, along with treatment strategies for tumors of the lacrimal gland are presented.
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Detection of Merkel cell polyomavirus with a tumour-specific signature in non-small cell lung cancer
Yumiko Hashida,Masayuki Imajoh,Yuiko Nemoto,Mikio Kamioka,Ayuko Taniguchi,Takahiro Taguchi,Motohiko Kume,Kazumasa Orihashi,Masanori Daibata +8 more
TL;DR: The expression of a viral oncoprotein, the presence of integrated MCPyV, and a truncated LT gene with a preserved retinoblastoma tumour-suppressor protein-binding domain in NSCLCs support the possibility that M CPyV is partly associated with the pathogenesis of NSCLC in a subset of patients.
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Human oncogenic viruses: nature and discovery.
TL;DR: Although individual human tumour viruses exert their malignant effects in different ways, there are common features that illuminate mechanisms of oncogenesis more generally, whether or not there is a viral aetiology.
Journal ArticleDOI
Human polyomaviruses and cancer: an overview.
José Carlos Mann Prado,T A Monezi,Aline T. Amorim,Vanesca de Souza Lino,Andressa Paladino,Enrique Boccardo +5 more
TL;DR: Present evidence only supports the role of MCPyV as a carcinogen to humans, and a summarized discussion on the current knowledge concerning the roleof MCPYV, TSPyV, JCPy V and BKPyV in human cancers is presented.
Journal ArticleDOI
Merkel cell carcinoma: histopathologic and prognostic features according to the immunohistochemical expression of Merkel cell polyomavirus large T antigen correlated with viral load
Valérie Leroux-Kozal,Nicolas Lévêque,Véronique Brodard,Candice Lesage,Oriane Dudez,Marc Makeieff,Lukshe Kanagaratnam,Marie-Danièle Diebold +7 more
TL;DR: Depending on MCPyV involvement, 2 MCC subgroups can be distinguished on histopathologic criteria, and the CM2B4 antibody is able to differentiate them reliably.
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