Journal ArticleDOI
Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.
About:
This article is published in Biochemistry.The article was published on 1974-07-30. It has received 1986 citations till now. The article focuses on the topics: Circular dichroism & Helix.read more
Citations
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Journal ArticleDOI
Using circular dichroism spectra to estimate protein secondary structure
TL;DR: This protocol details the basic steps of obtaining and interpreting CD data, and methods for analyzing spectra to estimate the secondary structural composition of proteins.
PatentDOI
Core structure of GP41 from the HIV envelope glycoprotein
TL;DR: The crystal structure of this complex, composed of the peptides N36 and C34, is a six-helical bundle that shows striking similarity to the low-pH-induced conformation of influenza hemagglutinin and likely represents the core of fusion-active gp41.
Journal ArticleDOI
Estimation of globular protein secondary structure from circular dichroism.
S W Provencher,J Glöckner +1 more
TL;DR: In this article, a linear combination of the CD spectra (from 190 to 240 nm) of 16 proteins whose secondary structures are known from X-ray crystallography was used to characterize helix, beta sheet, beta turn, and remainder.
Book ChapterDOI
Calculation of protein conformation from circular dichroism.
TL;DR: This chapter discusses some recent developments in the estimation of various conformations in a protein molecule from its circular dichroism (CD) spectrum in the ultraviolet region.
Journal ArticleDOI
Amphipathic, α‐helical antimicrobial peptides
TL;DR: This review considers alpha-helical, antimicrobial peptides from the point of view of six interrelated structural and physicochemical parameters that modulate their activity and specificity: sequence, size, structuring, charge, amphipathicity, and hydrophobicity.
References
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Journal ArticleDOI
A high resolution structure of an inhibitor complex of the extracellular nuclease of Staphylococcus aureus. I. Experimental procedures and chain tracing.
A. Arnone,Bier Cj,F. A. Cotton,Victor W. Day,E.E. Hazen,David S. Richardson,Jane S. Richardson,A. Yonath +7 more
TL;DR: The most significant feature of the nuclease structure is a large pocket which serves as the inhibitor binding site, and the lining of this pocket is revealed to be predominantly neutral or hydrophobic with the exception of several residues which specifically participate in binding the calcium ion and the nucleoside diphosphate.
Journal ArticleDOI
The use of computed optical rotatory dispersion curves for the evaluation of protein conformation.
Journal ArticleDOI
Optical activity of polypeptides and proteins.
TL;DR: The optical activity of a number of polypeptides and proteins has been calculated and it is concluded that the 222‐nm band of the α‐helix is a good method for detecting helices in proteins but that the 207‐and 191‐nm bands of the helix will not fit a linear superposition model.
Journal ArticleDOI
Improved Calculation of the nπ* Rotational Strength in Polypeptides
TL;DR: The calculation of the nπ* rotational strength in the α helix using the Kirkwood theory as extended by Moffitt and Tinoco agrees better with experiment than do previous theoretical values.
Journal ArticleDOI
Optical properties of polypeptides in the β‐conformation
TL;DR: The rotational strengths and oscillator strengths of the nπ* band and ππ* exciton bands have been calculated for antiparallel and parallel β-structures of varying length and width as mentioned in this paper.