Journal ArticleDOI
Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.
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This article is published in Biochemistry.The article was published on 1974-07-30. It has received 1986 citations till now. The article focuses on the topics: Circular dichroism & Helix.read more
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Structuring of H1 histone. Evidence of high-affinity binding sites for phosphate ions.
TL;DR: The defined and limited number of phosphate groups bound per histone molecule, the high affinity of the interaction and the effect on the structure of the histone suggest the participation of phosphate Groups in the binding of H1 histones to DNA.
Journal ArticleDOI
Comparison of lipid-dependent bilayer insertion of pHLIP and its P20G variant.
TL;DR: It is found that the P20G variant of the pH-Low Insertion Peptide has a higher helical propensity than the native pHLIP (pHLIP-WT), in both water:organic solvent mixtures and in the presence of lipid bilayers, and the deeply penetrating interfacial form (traditionally called State II) is populated only in pure phosphocholine bilayers.
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An Antibody Directed against the Fusion Peptide of Junín Virus Envelope Glycoprotein GPC Inhibits pH-Induced Membrane Fusion
Joanne York,Jody D. Berry,Ute Ströher,Ute Ströher,Qunnu Li,Heinz Feldmann,Heinz Feldmann,Min Lu,Meg Trahey,Jack H. Nunberg +9 more
TL;DR: A G2-directed monoclonal antibody, F100G5, is described that prevents membrane fusion by binding to an intermediate form of the protein on the fusion pathway, and studies indicate that F 100G5 identifies an on-path intermediate forms of GPC.
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A short proregion of trialysin, a pore‐forming protein of Triatoma infestans salivary glands, controls activity by folding the N‐terminal lytic motif
Rafael M. Martins,Rogerio Amino,Katia Regina Perez Daghastanli,Iolanda M. Cuccovia,Maria A. Juliano,Sergio Schenkman +5 more
TL;DR: It is shown here that trialysin is synthesized as a precursor that is less active than the protein released after saliva secretion, and a synthetic peptide flanked by a fluorophore and a quencher including the acidic proregion and the lytic N‐terminus of the protein is more active against cells and liposomes, increasing activity upon proteolysis.
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Phosphoribulokinase from Chlamydomonas reinhardtii: a Benson–Calvin cycle enzyme enslaved to its cysteine residues
TL;DR: A new mechanism for the PRK-GAPDH-CP12 formation involving a thiol disulfide exchange reaction with CP12 is uncovered and Cys16 of PRK is identified as a target of glutathionylation acting against oxidative stress.
References
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Journal ArticleDOI
Computed circular dichroism spectra for the evaluation of protein conformation
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Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion.
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Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 Angstrom resolution.
C. C. F. Blake,D. F. Koenig,D. F. Koenig,G. A. Mair,A. C. T. North,D. C. Phillips,V. R. Sarma +6 more
TL;DR: Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 A Resolution as mentioned in this paper, 3D Fourier synthesis at 2 a resolution.
Journal ArticleDOI
Structure of Myoglobin: A Three-Dimensional Fourier Synthesis at 2 Å. Resolution
J. C. Kendrew,R. E. Dickerson,B. E. Strandberg,R G Hart,D R Davies,D. C. Phillips,V. C. Shore +6 more