Journal ArticleDOI
Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.
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This article is published in Biochemistry.The article was published on 1974-07-30. It has received 1986 citations till now. The article focuses on the topics: Circular dichroism & Helix.read more
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Two-Phase Induction of the Nonnative α-Helical Form of β-Lactoglobulin in the Presence of Trifluoroethanol
TL;DR: In this paper, the trifluoroethanol-dependent induction of the nonnative α-helical form of β-lactoglubulin has been studied by circular dichroism spectroscopy Data analysis is performed by factor analysis and multivariate curve resolution.
Journal ArticleDOI
Cytotoxicity of pilosulin 1, a peptide from the venom of the jumper ant Myrmecia pilosula
Qi-xuan Wu,Malcolm A. King,Gregory R. Donovan,Dianne Alewood,Paul F. Alewood,William H. Sawyer,Brian A. Baldo,Brian A. Baldo +7 more
TL;DR: Analysis of pilosulin 1 using circular dichroism indicated that, in common with melittin and other Hymenoptera venom toxins, it had the potential to adopt an alpha-helical secondary structure.
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Glucagon conformation: use of optically detected magnetic resonance and phosphorescence of tryptophan to evaluate critical requirements for folding of the polypeptide chain.
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Fluorogenically active leucine zipper peptides as tag-probe pairs for protein imaging in living cells.
Hiroshi Tsutsumi,Wataru Nomura,Seiichiro Abe,Tomoaki Mino,Akemi Masuda,Nami Ohashi,Tomohiro Tanaka,Kenji Ohba,Naoki Yamamoto,Kazunari Akiyoshi,Hirokazu Tamamura +10 more
TL;DR: Small peptides, such as anoligohistidine tag (His tag), can be genetically incorporated into target proteins and used for purification of recombinant proteins, immobilization of proteins on microplates, and bioimaging of proteins at the surface of living cells with theirplementary partner molecules.
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Biophysical studies of the development of amyloid fibrils from a peptide fragment of cold shock protein B.
TL;DR: The concept that the ability to form amyloid fibrils is a generic property of polypeptide chains is supported, and that the mechanism of their formation is similar for different peptides and proteins.
References
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Journal ArticleDOI
Computed circular dichroism spectra for the evaluation of protein conformation
Journal ArticleDOI
Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion.
Journal ArticleDOI
Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 Angstrom resolution.
C. C. F. Blake,D. F. Koenig,D. F. Koenig,G. A. Mair,A. C. T. North,D. C. Phillips,V. R. Sarma +6 more
TL;DR: Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 A Resolution as mentioned in this paper, 3D Fourier synthesis at 2 a resolution.
Journal ArticleDOI
Structure of Myoglobin: A Three-Dimensional Fourier Synthesis at 2 Å. Resolution
J. C. Kendrew,R. E. Dickerson,B. E. Strandberg,R G Hart,D R Davies,D. C. Phillips,V. C. Shore +6 more