Journal ArticleDOI
Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.
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This article is published in Biochemistry.The article was published on 1974-07-30. It has received 1986 citations till now. The article focuses on the topics: Circular dichroism & Helix.read more
Citations
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Primary and secondary structural determinants in the receptor binding sequence beta-(38-57) from human luteinizing hormone.
TL;DR: The peptide appears not to require the disulfide bridge to retain a looped conformation with amphipathic secondary structure, and may contribute to a multicomponent receptor binding domain in hLH/hCG.
Journal ArticleDOI
Isolation and characterization of tropomyosin from fish muscle.
David H. Heeley,C. Hong +1 more
TL;DR: The fish tropomyosins were blocked at the N-terminus and, as expected, were found to be of similar amino acid composition, alpha-helical content and molecular weight to other vertebrate striated muscle forms.
Journal ArticleDOI
The reconstitution of denatured phosphoglycerate mutase.
TL;DR: The reconstitution of the tetrameric enzyme yeast phosphoglycerate mutase after denaturation in guanidine hydrochloride has been studied and complex kinetics indicating the rapid formation of structured monomers are revealed.
Journal ArticleDOI
A CD determination of the α-helix contents of the coat proteins of four filamentous bacteriophages: fd, IKe, Pf1, and Pf3
Beatrice A. Clack,Donald M. Gray +1 more
TL;DR: The α‐helix contents of these phages indicated that the coat proteins of the intact phages have CD spectra that are probably modeled better by the reference spectra of polypeptides than by those of globular proteins.
Journal ArticleDOI
A de Novo Designed Template for Generating Conformation-specific Antibodies That Recognize α-Helices in Proteins
Stephen M. Lu,Robert S. Hodges +1 more
TL;DR: A de novo designed parallel two-stranded α-helical coiled-coil template for immunization to prepare antibodies that recognize α- Helical protein sequences in the native protein to demonstrate the feasibility of this approach and demonstrate direct competition between peptide antigens and antibodies generated by the template.
References
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Journal ArticleDOI
Computed circular dichroism spectra for the evaluation of protein conformation
Journal ArticleDOI
Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion.
Journal ArticleDOI
Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 Angstrom resolution.
C. C. F. Blake,D. F. Koenig,D. F. Koenig,G. A. Mair,A. C. T. North,D. C. Phillips,V. R. Sarma +6 more
TL;DR: Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 A Resolution as mentioned in this paper, 3D Fourier synthesis at 2 a resolution.
Journal ArticleDOI
Structure of Myoglobin: A Three-Dimensional Fourier Synthesis at 2 Å. Resolution
J. C. Kendrew,R. E. Dickerson,B. E. Strandberg,R G Hart,D R Davies,D. C. Phillips,V. C. Shore +6 more