Journal ArticleDOI
Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.
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This article is published in Biochemistry.The article was published on 1974-07-30. It has received 1986 citations till now. The article focuses on the topics: Circular dichroism & Helix.read more
Citations
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The GCN4 bZIP targets noncognate gene regulatory sequences: quantitative investigation of binding at full and half sites.
TL;DR: Electrophoretic mobility shift assay (EMSA) and circular dichroism (CD) show that comparable levels of alpha-helical structure are induced in wt bZIP upon binding to cognate AP-1 or noncognate sites, and nonc Cognate sites may contribute to preorganization of stable protein structure before binding target DNA sites.
Journal ArticleDOI
Recombinant bovine heart mitochondrial F1-ATPase inhibitor protein: overproduction in Escherichia coli, purification, and structural studies.
G. Van Heeke,Lily Deforce,R. A. Schnizer,Regina Shaw,J. M. Couton,G. Shaw,Pill-Soon Song,Sheldon M. Schuster +7 more
TL;DR: Experimental evidence obtained by circular dichroism analyses supports this prediction and suggests F1I to be a highly stable, mainly alpha-helical protein which displays C-terminal alpha- helical coiled-coil intermolecular interaction.
Journal ArticleDOI
Resistance of lipophilin, a hydrophobic myelin protein, to denaturation by urea and guanidinium salts.
TL;DR: The combined evidence suggests that the extraordinary resistance of lipophilin to these denaturants is due to the presence of an impervious hydrophobic core.
Journal ArticleDOI
Hydrodynamic properties of D-beta-hydroxybutyrate dehydrogenase, a lipid-requiring enzyme.
Journal ArticleDOI
Role of aromatic residues in the structure-function relationship of alpha-bungarotoxin.
Yee Hsiung Chen,Yee Hsiung Chen,Jang Chyi Tai,Wan Jen Huang,Ming-Zong Lai,Mien Chie Hung,Ming Derg Lai,Jen Tsi Yang +7 more
TL;DR: The conformation of alpha-bungarotoxin and its cyanogen bromide cleaved and nitrated derivatives was studied by circular dichroism (CD) and indicates that Tyr-54 has a normal apparent dissociation constant and Tyr-24 ionizes at pH above 11.2.
References
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Journal ArticleDOI
Computed circular dichroism spectra for the evaluation of protein conformation
Journal ArticleDOI
Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion.
Journal ArticleDOI
Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 Angstrom resolution.
C. C. F. Blake,D. F. Koenig,D. F. Koenig,G. A. Mair,A. C. T. North,D. C. Phillips,V. R. Sarma +6 more
TL;DR: Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 A Resolution as mentioned in this paper, 3D Fourier synthesis at 2 a resolution.
Journal ArticleDOI
Structure of Myoglobin: A Three-Dimensional Fourier Synthesis at 2 Å. Resolution
J. C. Kendrew,R. E. Dickerson,B. E. Strandberg,R G Hart,D R Davies,D. C. Phillips,V. C. Shore +6 more