Journal ArticleDOI
Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.
About:
This article is published in Biochemistry.The article was published on 1974-07-30. It has received 1986 citations till now. The article focuses on the topics: Circular dichroism & Helix.read more
Citations
More filters
Journal ArticleDOI
Insights into the mechanism of heterodimerization from the 1H-NMR solution structure of the c-Myc-Max heterodimeric leucine zipper.
Pierre Lavigne,Matthew P. Crump,Stéphane M. Gagné,Robert S. Hodges,Cyril M. Kay,Brian D. Sykes +5 more
TL;DR: It has been shown that the LZ domains of the c-Myc and Max proteins specifically form a heterodimeric LZ at 20 degreesC and neutral pH, which suggests that theLZ domains are playing an important role in theheterodimerization of the corresponding gene products in vivo.
Journal ArticleDOI
Peptide:lipid ratio and membrane surface charge determine the mechanism of action of the antimicrobial peptide BP100. Conformational and functional studies
Mariana C. Manzini,Katia Regina Perez,Karin A. Riske,José Carlos Bozelli,Talita L. Santos,Marcia A. da Silva,Greice Kelle Viegas Saraiva,Mário José Politi,Ana P. Valente,Fabio C. L. Almeida,Hernan Chaimovich,Magali A. Rodrigues,Marcelo P. Bemquerer,Shirley Schreier,Iolanda M. Cuccovia +14 more
TL;DR: The cecropin-melittin hybrid antimicrobial peptide BP100 is selective for Gram-negative bacteria, negatively charged membranes, and weakly hemolytic, and at high salt, BP100-induced LUVS leakage requires higher peptide concentration, indicating that both electrostatic and hydrophobic interactions contribute to peptide binding.
Journal ArticleDOI
Characterization of a fatty acid-binding protein from rat heart.
TL;DR: Using a binding assay which measures the transfer of fatty acids between donor liposomes and protein, it was shown that both rat heart and liver fatty acid-binding proteins bind 2 mol of oleic acid or palmitic acid/mol of protein.
Journal ArticleDOI
Redox potential controls the structure and DNA binding activity of the paired domain.
Gianluca Tell,Andrea Scaloni,Lucia Pellizzari,Silvestro Formisano,Carlo Pucillo,Giuseppe Damante +5 more
TL;DR: This work demonstrates that the binding activity of the Prd domain is regulated through the oxidation/reduction of conserved cysteine residues, and proposes that this control mechanism should be involved in “switching” among different DNA sequences and therefore different target genes.
Journal ArticleDOI
A motif in human histidyl-tRNA synthetase which is shared among several aminoacyl-tRNA synthetases is a coiled-coil that is essential for enzymatic activity and contains the major autoantigenic epitope.
Nina Raben,Ralph C. Nichols,Jan Dohlman,Peter McPhie,Vaidehi Sridhar,C.Craig Hyde,Richard L. Leff,Paul H. Plotz +7 more
TL;DR: In this article, a recombinant histidyl-tRNA synthetase was found to be enzymatically active and recognized by human autoantibodies in the baculovirus system, and the peptides from this region (amino acids 1-60 and 1-47) have the predicted high alpha-helical content, but smaller fragments (1-30, 14-45, and 31-60) do not.
References
More filters
Journal ArticleDOI
Computed circular dichroism spectra for the evaluation of protein conformation
Journal ArticleDOI
Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion.
Journal ArticleDOI
Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 Angstrom resolution.
C. C. F. Blake,D. F. Koenig,D. F. Koenig,G. A. Mair,A. C. T. North,D. C. Phillips,V. R. Sarma +6 more
TL;DR: Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 A Resolution as mentioned in this paper, 3D Fourier synthesis at 2 a resolution.
Journal ArticleDOI
Structure of Myoglobin: A Three-Dimensional Fourier Synthesis at 2 Å. Resolution
J. C. Kendrew,R. E. Dickerson,B. E. Strandberg,R G Hart,D R Davies,D. C. Phillips,V. C. Shore +6 more