Journal ArticleDOI
Determination of the helix and beta form of proteins in aqueous solution by circular dichroism.
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This article is published in Biochemistry.The article was published on 1974-07-30. It has received 1986 citations till now. The article focuses on the topics: Circular dichroism & Helix.read more
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The refolding of denatured rabbit muscle creatine kinase
TL;DR: The refolding of rabbit muscle creatine kinase (ATP:creatine N-phosphotransferase, EC 2.7.2) which had been denatured in 3 M guanidine hydrochloride was monitored by studying the regain of enzyme activity and a pathway for the refolding process could be proposed.
Journal ArticleDOI
Stable proline box motif at the N-terminal end of alpha-helices.
A.R. Viguera,Luis Serrano +1 more
TL;DR: A novel N‐terminal α‐helix local motif that involves three hydrophobic residues and a Pro residue (Pro‐box motif) is described, and it is found that this motif is formed in peptides, and that the accompanyingHydrophobic interactions contribute up to 1.2 kcal/mol to helix stability.
Journal ArticleDOI
Synthetic and structural studies on Pyrularia pubera thionin: a single-residue mutation enhances activity against Gram-negative bacteria.
Miquel Vila-Perelló,Andrea Sánchez-Vallet,Francisco García-Olmedo,Antonio Molina,David Andreu +4 more
TL;DR: The thionin from Pyrularia pubera (Pp‐TH), a 47‐residue peptide with four internal disulfide bonds, was efficiently produced by chemical synthesis and showed a significant increase in antibiotic activity against several Gram‐negative bacteria, whereas it retained the same activity against other pathogens.
Journal ArticleDOI
Nuclear magnetic resonance evidence for the coexistence of several conformational states of rabbit cardiac and skeletal tropomyosins.
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Evidence for a coiled-coil interaction mode of disordered proteins from bacterial type III secretion systems.
Anastasia D. Gazi,Marina N. Bastaki,Spyridoula N. Charova,Eirini A. Gkougkoulia,Efthymios A. Kapellios,Nicholas J. Panopoulos,Michael Kokkinidis +6 more
TL;DR: Evidence from HrpO analogues from all T3 SS families and the flagellum suggests that the extreme flexibility and propensity for coiled-coil interactions of this diverse class of small, intrinsically disordered proteins might be important elements in the establishment of protein-protein interaction networks required for T3SS function.
References
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Journal ArticleDOI
Computed circular dichroism spectra for the evaluation of protein conformation
Journal ArticleDOI
Determination of the secondary structures of proteins by circular dichroism and optical rotatory dispersion.
Journal ArticleDOI
Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 Angstrom resolution.
C. C. F. Blake,D. F. Koenig,D. F. Koenig,G. A. Mair,A. C. T. North,D. C. Phillips,V. R. Sarma +6 more
TL;DR: Structure of Hen Egg-White Lysozyme: A Three-dimensional Fourier Synthesis at 2 A Resolution as mentioned in this paper, 3D Fourier synthesis at 2 a resolution.
Journal ArticleDOI
Structure of Myoglobin: A Three-Dimensional Fourier Synthesis at 2 Å. Resolution
J. C. Kendrew,R. E. Dickerson,B. E. Strandberg,R G Hart,D R Davies,D. C. Phillips,V. C. Shore +6 more