Development of defective and persistent Sendai virus vector: a unique gene delivery/expression system ideal for cell reprogramming.
Ken Nishimura,Masayuki Sano,Manami Ohtaka,Birei Furuta,Yoko Umemura,Yoshiro Nakajima,Yuzuru Ikehara,Toshihiro Kobayashi,Hiroaki Segawa,Satoko Takayasu,Hideyuki Sato,Kaori Motomura,Eriko Uchida,Toshie Kanayasu-Toyoda,Makoto Asashima,Hiromitsu Nakauchi,Teruhide Yamaguchi,Mahito Nakanishi +17 more
TLDR
A novel replication-defective and persistent Sendai virus (SeVdp) vector based on a noncytopathic variant virus, which fulfills all of the requirements for cell reprogramming and for stem cell research is reported.About:
This article is published in Journal of Biological Chemistry.The article was published on 2011-02-11 and is currently open access. It has received 347 citations till now. The article focuses on the topics: Reprogramming & Induced pluripotent stem cell.read more
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A decade of transcription factor-mediated reprogramming to pluripotency
TL;DR: The mechanisms underlying transcription factor-mediated reprogramming are still poorly understood; however, several mechanistic insights have recently been obtained, making it more amenable to applications in the fields of regenerative medicine, disease modelling and drug discovery.
Journal ArticleDOI
Induced pluripotent stem cells — opportunities for disease modelling and drug discovery
TL;DR: How iPSCs are being exploited to illuminate disease pathophysiology, identify novel drug targets and enhance the probability of clinical success of new drugs is discussed.
Journal ArticleDOI
Generation of Rejuvenated Antigen-Specific T Cells by Reprogramming to Pluripotency and Redifferentiation
Toshinobu Nishimura,Shin Kaneko,Ai Kawana-Tachikawa,Yoko Tajima,Haruo Goto,Dayong Zhu,Kaori Nakayama-Hosoya,Shoichi Iriguchi,Yasushi Uemura,Takafumi Shimizu,Naoya Takayama,Daisuke Yamada,Ken Nishimura,Manami Ohtaka,Nobukazu Watanabe,Satoshi Takahashi,Aikichi Iwamoto,Haruhiko Koseki,Mahito Nakanishi,Koji Eto,Hiromitsu Nakauchi +20 more
TL;DR: This work reprogrammed clonally expanded antigen-specific CD8(+) T cells from an HIV-1-infected patient to pluripotency and found that this method can be effective for generating specific T-cells for other pathology-associated antigens.
Journal ArticleDOI
Turning straw into gold: directing cell fate for regenerative medicine
Dena E. Cohen,Douglas A. Melton +1 more
TL;DR: Two main approaches to accomplishing cell-replacement therapy are reviewed: in vitro directed differentiation, which is used to push pluripotent stem cells, including embryonic stem cells or induced pluripOTS, through steps similar to those that occur during embryonic development; and reprogramming, in which a differentiated cell is converted directly into the cell of interest without proceeding through a pluripotency intermediate.
PatentDOI
Induced pluripotent stem cells
Shinya Yamanaka,伸弥 山中 +1 more
TL;DR: This chapter introduces an overview of direct reprogramming with defined factors as well as a special focus on its potentials and challenges.
References
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Journal ArticleDOI
Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors.
TL;DR: Induction of pluripotent stem cells from mouse embryonic or adult fibroblasts by introducing four factors, Oct3/4, Sox2, c-Myc, and Klf4, under ES cell culture conditions is demonstrated and iPS cells, designated iPS, exhibit the morphology and growth properties of ES cells and express ES cell marker genes.
Journal ArticleDOI
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
TL;DR: 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
Journal ArticleDOI
Pattern Recognition Receptors and Inflammation
Osamu Takeuchi,Shizuo Akira +1 more
TL;DR: The role of PRRs, their signaling pathways, and how they control inflammatory responses are discussed.
Journal ArticleDOI
Generation of germline-competent induced pluripotent stem cells
TL;DR: iPS cells competent for germline chimaeras can be obtained from fibroblasts, but retroviral introduction of c-Myc should be avoided for clinical application.
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