Distribution of microRNA biomarker candidates in solid tissues and body fluids
TLDR
The Human miRNA Tissue Atlas is reported, and the web-based repository now hosting 982 full miRNomes all of which are measured by the same microarray technology, identified miRNAs that are rather specific for tissues.Abstract:
Small non-coding RNAs, especially microRNAs, are discussed as promising biomarkers for a substantial number of human pathologies. A broad understanding in which solid tissues, cell types or body fluids a microRNA is expressed helps also to understand and to improve the suitability of miRNAs as non- or minimally-invasive disease markers. We recently reported the Human miRNA Tissue Atlas ( http://www.ccb.uni-saarland.de/tissueatlas ) containing 105 miRNA profiles of 31 organs from 2 corpses. We subsequently added miRNA profiles measured by others and us using the same array technology as for the first version of the Human miRNA Tissue Atlas. The latter profiles stem from 163 solid organs including lung, prostate and gastric tissue, from 253 whole blood samples and 66 fractioned blood cell isolates, from body fluids including 72 serum samples, 278 plasma samples, 29 urine samples, and 16 saliva samples and from different collection and storage conditions. While most miRNAs are ubiquitous abundant in solid tissues and whole blood, we also identified miRNAs that are rather specific for tissues. Our web-based repository now hosting 982 full miRNomes all of which are measured by the same microarray technology. The knowledge of these variant abundances of miRNAs in solid tissues, in whole blood and in other body fluids is essential to judge the value of miRNAs as biomarker.read more
Citations
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Journal ArticleDOI
Trends in the development of miRNA bioinformatics tools.
TL;DR: Five key trends were observed: miRNA identification and target prediction have been hot spots in the past decade; manual curation and TM are the main methods for collecting miRNA knowledge from literature; most early tools are well maintained and widely used; however, novel ones have begun to emerge and disease-associated miRNA tools are emerging.
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Total Extracellular Small RNA Profiles from Plasma, Saliva, and Urine of Healthy Subjects.
Ashish Yeri,Amanda Courtright,Rebecca Reiman,Elizabeth Carlson,Taylor Beecroft,Alex Janss,Ashley L. Siniard,Ryan Richholt,Chris Balak,Joel Rozowsky,Robert R. Kitchen,Elizabeth Hutchins,Joseph Winarta,Roger McCoy,Matthew Anastasi,Seungchan Kim,Matthew J. Huentelman,Kendall Van Keuren-Jensen +17 more
TL;DR: A systematic analysis of small exRNAs present in each biofluid, as well as an analysis of exogenous RNAs are provided, finding that a large number of RNA fragments in plasma and urine have sequences that are assigned to YRNA and tRNA fragments respectively.
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MicroRNA in diagnosis and therapy monitoring of early-stage triple-negative breast cancer
Mustafa Kahraman,Anne Röske,Thomas Laufer,Tobias Fehlmann,Christina Backes,Fabian Kern,Jochen Kohlhaas,Hannah Schrörs,Anna Saiz,Cassandra Zabler,Nicole Ludwig,Peter A. Fasching,Reiner Strick,Matthias Rübner,Matthias W. Beckmann,Eckart Meese,Andreas Keller,Michael G. Schrauder +17 more
TL;DR: It is reported that miRNA expression measured from blood facilitates early and minimally-invasive diagnosis of basal-like TNBC and that blood-borne miRNA profiles monitored over time have potential to predict pathological complete response (pCR).
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Circulating miRNA analysis for cancer diagnostics and therapy.
TL;DR: This review provides a comprehensive overview of circulating miRNA analysis and highlights the importance of sampling and quality control, the technical aspects of miRNA extraction and quantification, recommendations for downstream analysis and conclude with future perspectives.
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