Journal ArticleDOI
Human embryonic stem cell-derived retinal pigment epithelium in patients with age-related macular degeneration and Stargardt's macular dystrophy: follow-up of two open-label phase 1/2 studies
Steven D. Schwartz,Carl D. Regillo,Byron L. Lam,Dean Eliott,Philip J. Rosenfeld,Ninel Z. Gregori,Jean-Pierre Hubschman,Janet L. Davis,Gad Heilwell,Marc J. Spirn,Joseph I. Maguire,Jane Bateman,Rosaleen Ostrick,Debra Morris,Matthew Vincent,Eddy Anglade,Lucian V. Del Priore,Robert Lanza +17 more
TLDR
The results suggest that hESC-derived cells could provide a potentially safe new source of cells for the treatment of various unmet medical disorders requiring tissue repair or replacement.About:
This article is published in The Lancet.The article was published on 2015-02-07. It has received 990 citations till now. The article focuses on the topics: Macular dystrophy & Stargardt disease.read more
Citations
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Survival of an HLA-mismatched, bioengineered RPE implant in dry age-related macular degeneration
Amir H. Kashani,Jane S. Lebkowski,David R. Hinton,Danhong Zhu,Mohamed A. Faynus,Sanford Chen,Firas M. Rahhal,Robert L. Avery,Hani Salehi-Had,Clement Wn Chan,Neal V. Palejwala,April Ingram,Wei Dang,Chih-Min Lin,Debbie Mitra,Juan-Carlos Martinez-Camarillo,J. Bailey,Cassidy C. Arnold,Britney O Pennington,Narsing A. Rao,Lincoln V. Johnson,Dennis O. Clegg,Mark S. Humayun +22 more
TL;DR: In this article , the safety and preliminary efficacy of a subretinal implant consisting of a polarized monolayer of allogeneic human embryonic stem cell-derived retinal pigmented epithelium (RPE) cells in subjects with geographic atrophy (GA) secondary to dry age-related macular degeneration was evaluated.
Journal ArticleDOI
A strategy to ensure safety of stem cell- derived retinal pigment epithelium cells
Parul Choudhary,Paul Whiting +1 more
TL;DR: A novel strategy for ensuring the absence of stem cells in the RPE population is presented and it is demonstrated that flow sorting on the basis of CD59 expression can effectively purify RPE and deplete stem cells, resulting in a population free from stem cell impurity.
Journal ArticleDOI
Wogonin induces retinal neuron-like differentiation of bone marrow stem cells by inhibiting Notch-1 signaling.
TL;DR: Wogonin appears to promote retinal neuron-like differentiation of BMSCs by antagonizing the inhibitory actions of Notch-1 signaling on neurogenesis and may be useful in the treatment of retinal degenerative diseases.
Journal ArticleDOI
Brain Organoids: Tiny Mirrors of Human Neurodevelopment and Neurological Disorders.
Anuradha Yadav,Anuradha Yadav,Brashket Seth,Brashket Seth,Rajnish Kumar Chaturvedi,Rajnish Kumar Chaturvedi +5 more
TL;DR: The contemporary state of brain organoids is presented with special emphasis on their mode of generation and their utility in modelling neurological disorders, drug discovery, and clinical trials.
Journal ArticleDOI
Xeno-free and feeder-free culture and differentiation of human embryonic stem cells on recombinant vitronectin-grafted hydrogels
Li Hua Chen,Tzu Cheng Sung,Henry Hsin-Chung Lee,Akon Higuchi,Huan Chiao Su,Kuan Ju Lin,Yu Ru Huang,Qing Dong Ling,S. Suresh Kumar,Abdullah A. Alarfaj,Murugan A. Munusamy,Michiyo Nasu,Da Chung Chen,Shih Tien Hsu,Yung Chang,Kuei Fang Lee,Han Chow Wang,Akihiro Umezawa +17 more
TL;DR: Hydrogels developed by adjusting surface charge of the hydrogels with grafting of poly-l-lysine for optimal culture of human embryonic stem cells differentiated into cardiomyocytes under xeno-free culture conditions with much higher efficiency than conventional recombinant vitronectin or Matrigel-coating dishes.
References
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Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Kazutoshi Takahashi,Koji Tanabe,Mari Ohnuki,Megumi Narita,Tomoko Ichisaka,Kiichiro Tomoda,Shinya Yamanaka +6 more
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TL;DR: Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages.
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Establishment in culture of pluripotential cells from mouse embryos
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Journal ArticleDOI
The Retinal Pigment Epithelium in Visual Function
TL;DR: This review summarizes the current knowledge of RPE functions and describes how failure of these functions causes loss of visual function.
Journal ArticleDOI
Safety and Efficacy of Gene Transfer for Leber's Congenital Amaurosis
Albert M. Maguire,Francesca Simonelli,Eric A. Pierce,Edward N. Pugh,Federico Mingozzi,Jeannette L. Bennicelli,Sandro Banfi,Kathleen A. Marshall,Francesco Testa,Enrico Maria Surace,Settimio Rossi,Arkady Lyubarsky,Valder R. Arruda,Barbara A. Konkle,Edwin M. Stone,Edwin M. Stone,Junwei Sun,Jonathan B. Jacobs,L. F. Dell'Osso,Richard W. Hertle,Jian Xing Ma,T. Michael Redmond,Xiaosong Zhu,Bernd Hauck,Olga Zelenaia,Kenneth S. Shindler,Maureen G. Maguire,J. Fraser Wright,Nicholas J. Volpe,Jennifer Wellman McDonnell,Alberto Auricchio,Katherine A. High,Katherine A. High,Jean Bennett +33 more
TL;DR: This study investigated the safety of subretinal delivery of a recombinant adeno-associated virus (AAV) carrying RPE65 complementary DNA (cDNA) and found three patients with LCA2 had an acceptable local and systemic adverse-event profile after delivery of AAV2.hRPE65v2.
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