Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance.
TLDR
Branched-chain fatty acids of the iso and anteiso series occur in many bacteria as the major acyl constituents of membrane lipids and are an important criterion used to aid identification and classification of bacteria.About:
This article is published in Microbiological Research.The article was published on 1991-06-01 and is currently open access. It has received 1303 citations till now. The article focuses on the topics: Fatty acid & Polyunsaturated fatty acid.read more
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Journal ArticleDOI
Discovery of a metagenome-derived enzyme that produces branched-chain acyl-(acyl-carrier-protein)s from branched-chain α-keto acids.
Jeffrey W. Craig,Sean F. Brady +1 more
TL;DR: Results from a series of heterologous expression experiments in Burkholderia graminis C4D1M show that nasB functions to provide branched-chain acyl-ACPs for nasA, and hypothesized that this enzyme was likely to be involved in the formation of ACP-linked fatty acids similar to those produced by bacterial FAS II systems.
Journal ArticleDOI
Microbial production of bi-functional molecules by diversification of the fatty acid pathway.
Shivani Garg,Ludmila Rizhsky,Huanan Jin,Xiaochen Yu,Fuyuan Jing,Marna D. Yandeau-Nelson,Basil J. Nikolau +6 more
TL;DR: This study unveils the biocatalytic potential of KASIII for synthesizing diverse ω-functionalized fatty acids and demonstrates the in vivo diversion of FAS to produce novelπ�-1 hydroxy-branched fatty acids from glucose in two bioengineered microbial hosts.
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Plasticity of Coagulase-Negative Staphylococcal Membrane Fatty Acid Composition and Implications for Responses to Antimicrobial Agents
TL;DR: Preformed fatty acids can replace biosynthesized fatty acids in the glycerolipids of coagulase-negative staphylococci, and indicates that bacterial fatty acid biosynthesis system II may not be a good target for antimicrobial agents in these organisms.
Journal ArticleDOI
Gas chromatographic analysis to compare the fatty acid composition of fifteen lichen species, with a focus on Stereocaulon
Thi Huyen Vu,Daniel Catheline,David Delmail,Joël Boustie,Philippe Legrand,Françoise Lohézic-Le Dévéhat +5 more
Abstract: The composition of fatty acids (FAs) is known to discriminate families within genera of bacteria, fungi, octocorals and algae. Here, we applied the more sensitive gas chromatographic (GC) analysis to investigate the taxonomic value of using FA composition to discriminate 15 species of lichen, including three chlorolichens (two Cladonia species and Stereocaulon scutelligerum ), nine tripartite lichens ( Stereocaulon species) and three cyanolichens ( Lichina pygmaea , Collema cristatum , Peltigera membranacea ). One macroscopic cyanobacterium ( Nostoc sp.), corresponding to the photobiont partner of Peltigera and Collema cyanolichens, was included for comparison. Five lipid extraction methods were evaluated, using Stereocaulon scutelligerum . Shaking at 80 °C with chloroform/methanol (2:1, v/v) was the most efficient and reproducible method. The total FA composition of the 16 species was compared, using GC coupled with flame ionization detection or mass spectrometry. A statistical hierarchical cluster analysis was used to determine the similarity between the FA profiles, based on total, saturated, unsaturated and branched-chain FAs (BCFAs). The BCFA profile allowed a distinction between the Stereocaulon species and the cyanolichens, in contrast to all the other types of FAs. A detailed characterization of eight FA subclasses, provided by the GC analysis, suggested a preferential pathway for the biogenesis of unsaturated FAs through desaturases, which was especially favoured in the cyanolichens.
Dissertation
Organic carbon transformation in agricultural soils : radiocarbon analysis of organic matter fractions and biomarker compounds
TL;DR: In this article, a contamination of the soil at the study site at Halle/Saale (Germany) with fossil, lignite-derived carbon was identified by high apparent 14C ages of the organic matter in the plough horizon of about 5000 years BP and a decrease in 14C age with increasing soil depth.
References
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Fatty Acid Synthesis and Its Regulation
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Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa
TL;DR: Two-dimensional thin-layer chromatography showed that almost identical and very characteristic polar lipid patterns were given by all the organisms under study: the only major components were diphosphatidylglycerol, phosph atidylinositol and two phospho-glycolipids chromatographing similarly to, but distinguishable from, the mono- and diacyl phosphatidolinositols characteristic of Nocardia and other actinomycetes.
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Lipids in the Classification and Identification of Coryneform Bacteria Containing Peptidoglycans Based on 2, 4-diaminobutyric Acid
M. D. Collins,Dorothy Jones +1 more
TL;DR: The results of the present study indicate that lipid markers may be of considerable value in the classification and identification of 2, 4-diaminobutyric acid-containing phytopathogenic and saprophytic coryneform bacteria.
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The biology and enzymology of eukaryotic protein acylation
TL;DR: The N-Myristoylated Protein s Have Diff erent Intracellular Destinations and the Importance of Sequ ence Context is illustrated.