Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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In vitro stimulation of articular chondrocyte differentiated function by 1,25-dihydroxycholecalciferol or 24R,25-dihydroxycholecalciferol.
TL;DR: Results suggest that both 1,25‐(OH)2D3 and 24R,25-dihydroxycholecalciferol act specifically on articular chondrocytes to promote phenotype expression.
Journal ArticleDOI
Proteoglycan alterations during developing experimental osteoarthritis in a novel hip joint model.
TL;DR: The data indicate that proteoglycans are fragmented by proteolytic cleavage and lost from the cartilage, and are smaller and have lost the ability to aggregate with hyaluronic acid in knee joint osteoarthritis.
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Type II collagen from lathyritic rat chondrosarcoma: preparation and in vitro fibril formation.
Sandra L. Lee,Karl A. Piez +1 more
TL;DR: The continued presence of thin filaments when type II fibril formation was complete suggests either that filaments are in slow equilibrium with fibrils or that they may not be intermediates but rather a separate product.
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Monomeric and aggregate proteoglycans in the chondrogenic differentiation of embryonic chick limb buds.
N. S. Vasan,J. W. Lash +1 more
TL;DR: It is suggested that the appearance of the aggregate formation is also due to synthesis of a stable hyaluronic acid binding region of the core protein.
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Characterization of the human gene that encodes the peptide core of secretory granule proteoglycans in promyelocytic leukemia HL-60 cells and analysis of the translated product.
TL;DR: Based upon the deduced amino acid sequence of a cDNA that had been proposed to encode the peptide core of an eosinophil and a HL-60 cell secretory granule proteoglycan, a 16-amino acid peptide was synthesized and used to elicit rabbit antibodies for study of the translation and post-translational modification of this gene product in hematopoietic cells.
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.