Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Experimental transplantation of the Swarm rat chondrosarcoma into bone: radiological and pathological studies.
TL;DR: The natural history of the tumor when implanted into the bone of the rat, and its histological growth pattern with its radiological appearance are described, showed that the tumor grows slowly in the bone.
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Decrease in cytosolic free Ca2+ and enhanced proteoglycan synthesis induced by cartilage derived growth factors in cultured chondrocytes.
Yael Eilam,A. Beit-Or,Zvi Nevo +2 more
TL;DR: The results may indicate that whereas cell proliferation in chondrocytes is signaled by an increase in cytoplasmic Ca2+ ([Ca2+]in), proteoglycan synthesis is signaling by a decrease in [Ca2 +]in.
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Proteoglycan structure and metabolism in normal and osteoarthritic cartilage of guinea pigs.
TL;DR: Two species of proteoglycans that exhibited the same electrophoretic mobilities in agarose/acrylamide gels, the same elution profiles on high performance liquid chromatography, and the same galactosamine/glucosamine ratios were found in all normal and osteoarthritic cartilage specimens tested.
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Immunological analysis of proteoglycan structural changes in the early stage of experimental osteoarthritic canine cartilage lesions.
TL;DR: Specific modifications of the proteoglycan (PG) structure of osteoarthritic dog cartilage in relation to endogenous metalloprotease activity were examined using murine anti‐proteoglycan monoclonal antibodies (MoAbs).
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The effects of dansylation and acetylation on the interaction between hyaluronic acid and the hyaluronic acid-binding region of cartilage proteoglycans.
TL;DR: The results indicate that there are separate sites in the HA-binding region molecules which are protected from dansylation when both hyaluronic acid and the link protein are present or when only hyaluonic acid is present, suggesting that the HA -binding region of the proteoglycan molecule interacts with the linkprotein as well as with hyalURonic acid in forming proteogly can aggregates.
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.