Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Aggregation of Proteoglycans in Cephalic, Epiphyseal and Articular Cartilages in Growing Sheep
TL;DR: During the first 2 months of postnatal development the proportion of proteoglycans isolated as aggregates tripled in the articular and mandibular condylar cartilage, it decreased substantially in the anterior and posterior nasal septum, while it decreased slightly in the other cartilages.
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Somite chondrogenesis: alterations in cyclic AMP levels and proteoglycan synthesis.
TL;DR: The results indicate that a decrease in cAMP levels may be necessary for chondrogenic expression in somites, and the aggregation of proteoglycans was analyzed by gel chromatography and found to increase with a corresponding decrease incAMP levels.
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Chondroitin sulphate proteoglycans in the vitreous gel of sheep and goat.
TL;DR: The findings showed that, firstly, the CS content and the hydrodynamic size of the CS chain and the sulphation pattern of CS chains from versican and collagen IX in both sheep and goat vitreous gels are PG type-dependent.
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Ulcerative colitis and familial polyposis oncologic transformation to colon carcinoma: Changes in carcinoembryonic antigen release
TL;DR: The extracellular matrix from malignant colon cells contains agents that support colon cell oncogenic transformation, including glycoproteins resistant to collagenase action.
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The epidermal growth factor-like domain of the large proteoglycans from articular cartilage (aggrecans). Estimate of content at different ages and in osteoarthritis
TL;DR: Variations in the amount of newly synthesized aggrecan, in the proteolysis of the carboxy terminal domain of aggrecans and in the alternating splicing of the EGF-like domain might explain the results shown here.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.