Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Journal ArticleDOI
A murine tumor producing a matrix of basement membrane
TL;DR: A murine tumor previously classified as a poorly differentiated chondrosarcoma is studied, and it is shown at the ultrastructural level that the tumor matrix is a homogeneous, nonfibrillar material, resembling basement membrane.
Journal ArticleDOI
Culture and growth characteristics of chondrocytes encapsulated in alginate beads.
TL;DR: Preliminary studies with collagen and chitosan matrixes encapsulated in "hollow" beads suggest that cell growth and morphology are profoundly influenced by the composition of the cellular environment.
Journal ArticleDOI
Isolation and characterization of two sialoproteins present only in bone calcified matrix.
A Franzén,D Heinegård +1 more
TL;DR: Antigenicity of sialoprotein II was not affected by reduction and alkylation, was only partially lost upon trypsin digestion and was completely lost upon fragmentation of the core protein by alkaline-borohydride treatment, indicating that all antigenic sites were located in the protein portion.
Book ChapterDOI
Structure and Metabolism of Connective Tissue Proteoglycans
TL;DR: The segregation of the proteoglycans into a separate category is based on a few specific characteristics, including the fact that the d-glucuronic-acid-containing repeating disaccharide of chondroitin, N-acetylchondrosine, has recently been identified as a component of thyroglobulin.
Journal ArticleDOI
Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibodies to cartilage proteoglycan
TL;DR: The 1/20/5-D-4 monoclonal antibody appears to recognize a common determinant in their polysaccharide moieties, consistent with several biochemical analyses showing the absence of keratan sulfate in proteoglycan synthesised by this tissue.
References
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Journal ArticleDOI
Structure of the Neutral Trisaccharide of the Chondroitin 4-Sulfate-Protein Linkage Region
Lennart Rodén,Rita Smith +1 more
TL;DR: The following structure is proposed for the carbohydrate sequence in the chondroitin 4-sulfate-protein linkage region of bovine nasal septum: O-β-d-galactopyranosyl-(1 → 4)-d-xylose, respectively.
Journal ArticleDOI
Characteristics of the protein-keratan sulfate core and of keratan sulfate prepared from bovine nasal cartilage proteoglycan.
Vincent C. Hascall,Rick Riolo +1 more
TL;DR: The data suggest that the core molecules contain a protein (or combination of proteins) with a molecular weight of about 200,000 to which different amounts of keratan sulfate are attached, and that polysaccharide molecules with larger molecular weights have higher ratios of glucosamine to galactosamine.
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Characterization of chondroitin sulfate isolated from trypsin-chymotrypsin digests of cartilage proteoglycans
TL;DR: The data in sum indicate that peptide fragments which contain from 1 to 8 polysaccharide chains are released when the proteoglycans are digested with trypsin-chymotrypsin digestion, which indicates that some peptides contain large numbers of keratan sulfate chains.
Journal ArticleDOI
Cathepsins B1 and D. Action on human cartilage proteoglycans
TL;DR: It is concluded that the results are consistent with both cathepsins B1 and D playing a part in the catabolism of cartilage matrix.
Journal ArticleDOI
Comparative Biochemistry of Keratosulfates
Martin B. Mathews,J.A. Cifonelli +1 more