Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Biosynthesis of proteoglycans and their assembly into aggregates in cultures of chondrocytes from the Swarm rat chondrosarcoma.
TL;DR: In this paper, the authors measured the movement of newly synthesized proteoglycans from inside the cells into the extracellular matrix and, finally, into the culture medium by measuring the distribution of 35S-labeled proteoglycan in the medium, a 4 M guanidine HCl extract of the cell layer, and in the remaining residue for a number of chase times following a 5-min pulse with [35S]sulfate.
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Biosynthesis of collagen and other matrix proteins by articular cartilage in experimental osteoarthrosis
TL;DR: Osteoarthrosis was induced in one knee joint of dogs by an established surgical procedure and changes in the articular cartilage in the biosynthesis of collagen and other proteins were sought by radiochemical labelling in vivo.
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Proteoglycan changes during restoration of transparency in corneal scars
TL;DR: The results showed that initially opaque scars which contained the large interfibrillar spaces also contained unusually large chondroitin sulfate proteoglycans with glycosaminoglycan side chains of normal size, suggesting that the corneal stromal proteoglyCans may play a fundamental role in regulating cornesal collagen fibril spacing.
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Characterization of bovine aorta proteoglycan extracted with guanidine hydrochloride in the presence of protease inhibitors.
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Two Distinct Chondroitin Sulfate ABC Lyases AN ENDOELIMINASE YIELDING TETRASACCHARIDES AND AN EXOELIMINASE PREFERENTIALLY ACTING ON OLIGOSACCHARIDES
Akio Hamai,Nobukazu Hashimoto,Hideo Mochizuki,Fumikazu Kato,Yoshitaka Makiguchi,Katsuyuki Horie,Sakaru Suzuki +6 more
TL;DR: The results show that the known ability of the conventional enzyme called “chondroitinase ABC” to catalyze the complete depolymerization of chondroit in sulfates to unsaturated disaccharides may actually result from the combination reactions by endoeliminase and exoelIMinase (chONDroitin sulfate ABC exolyase).
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.