Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Chymopapain, chemonucleolysis, and nucleus pulposus regeneration.
TL;DR: It is suggested that the nucleus can regenerate following the injection of chymopapain, as demonstrated by [35S]sulphate-labeling and proteoglycan isolation, which demonstrated the nucleus pulposus retained the ability to synthesize proteoglycans, but these were degraded by endogenous proteolytic activity.
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The effect of cycloheximide on synthesis of proteoglycans by cultured chondrocytes from the Swarm rat chondrosarcoma.
TL;DR: Results suggest that after cycloheximide treatment, the rate of [35S]sulfate incorporation into proteoglycan, after a correction for increases in chondroitin sulfate chain size, was directly proportional to the size of the intracellular pool of core protein.
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Specific binding of bone sialoprotein to Staphylococcus aureus isolated from patients with osteomyelitis
Cecilia Rydén,Alia Yacoub,Ingemar Maxe,Dick Heinegård,Åke Oldberg,Ahnders Franzén,Åsa Ljungh,Kristofer Rubin +7 more
TL;DR: Results indicate that the major staphylococcal-binding site resides in the bone sialoprotein core protein and not in the carbohydrate side chains and no inhibition of bone sIALoprotein binding could be detected for whole human serum or purified plasma proteins.
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Assembly of newly synthesized proteoglycan and link protein into aggregates in cultures of chondrosarcoma chondrocytes.
TL;DR: The data suggest that the formation of proteoglycan aggregates may be a sequential process in which a link.monomer complex forms and this complex subsequently binds to hyaluronic acid.
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Critical Roles of Glycosaminoglycan Side Chains of Cartilage Proteoglycan (Aggrecan) in Antigen Recognition and Presentation
Tibor T. Glant,Edit I. Buzás,Alison Finnegan,Gabriela Negroiu,Gabriella Cs-Szabó,Gabriella Cs-Szabó,Katalin Mikecz,Katalin Mikecz +7 more
TL;DR: Proteoglycan-induced progressive polyarthritis is dictated by three major components: genetic background of the BALB/c strain, highly specific T cell response to epitope(s) masked by a KS chain in aging tissue, and the presence of proteoglycan (CS stub)-specific B cells required for sufficient Ag presentation.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.