Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Journal ArticleDOI
A murine tumor producing a matrix of basement membrane
TL;DR: A murine tumor previously classified as a poorly differentiated chondrosarcoma is studied, and it is shown at the ultrastructural level that the tumor matrix is a homogeneous, nonfibrillar material, resembling basement membrane.
Journal ArticleDOI
Culture and growth characteristics of chondrocytes encapsulated in alginate beads.
TL;DR: Preliminary studies with collagen and chitosan matrixes encapsulated in "hollow" beads suggest that cell growth and morphology are profoundly influenced by the composition of the cellular environment.
Journal ArticleDOI
Isolation and characterization of two sialoproteins present only in bone calcified matrix.
A Franzén,D Heinegård +1 more
TL;DR: Antigenicity of sialoprotein II was not affected by reduction and alkylation, was only partially lost upon trypsin digestion and was completely lost upon fragmentation of the core protein by alkaline-borohydride treatment, indicating that all antigenic sites were located in the protein portion.
Book ChapterDOI
Structure and Metabolism of Connective Tissue Proteoglycans
TL;DR: The segregation of the proteoglycans into a separate category is based on a few specific characteristics, including the fact that the d-glucuronic-acid-containing repeating disaccharide of chondroitin, N-acetylchondrosine, has recently been identified as a component of thyroglobulin.
Journal ArticleDOI
Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibodies to cartilage proteoglycan
TL;DR: The 1/20/5-D-4 monoclonal antibody appears to recognize a common determinant in their polysaccharide moieties, consistent with several biochemical analyses showing the absence of keratan sulfate in proteoglycan synthesised by this tissue.
References
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Electron microscopic studies of proteoglycan aggregates from bovine articular cartilage.
TL;DR: The stoichiometry of binding ofSubunits to filament, calculated from the data reported here, is close to that for the binding of subunits to hyaluronic acid reported by others.
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Ageing of human cartilage.
TL;DR: A study of the distribution of chondroitin sulphate and keratosulphate in rib cartilage of various age-groups is suggested and a shift from D-galactosamine to glucosamine with increasing age in epiphyseal human cartilage is demonstrated.
Journal ArticleDOI
Macromolecular models of proteinpolysaccharides from bovine nasal cartilage based on electron microscopic studies.
TL;DR: A model of increasing complexity has been derived for the macromolecular organization of cartilage ground substance and suggests that both PPL3 and PGS may exist as mixtures of monomers and dimers formed by the end to end association of monomer.
Journal ArticleDOI
Variations in keratosulfates.
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Treatment of bovine nasal cartilage proteoglycan with chondroitinases from Flavobacterium heparinum and Proteus vulgaris.
TL;DR: The results indicate that the noncovalent interactions which are essential for the aggregation of proteoglycans do not require the presence of the chondroitin sulfate portion of the macromolecules.