Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
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Stereological studies on the epiphyseal growth cartilage and characterization of costal cartilage proteoglycans in the achondroplastic (cn/cn) mouse.
TL;DR: The achondroplastic mouse is a dwarf mouse in which the endochondral growth of the skeleton is disturbed and the main morphological characteristic of the affected growth cartilage is an underdeveloped hypertrophic zone.
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Physical and immunochemical properties of low-buoyant-density proteoglycans from avian cartilage.
TL;DR: The results suggest that the low-buoyant-density proteoglycans found in the link (A 1 D 5 ) fraction from avian cartilage contain some of the antigenic determinants found on the proteoglycan monomer.
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Physicochemical properties of cartilage proteoglycans extracted by lanthanum chloride
TL;DR: Proteoglycan subunits (A1D1) prepared from PLaCl3 showed identical properties to those obtained from other high ionic strength cartilage extracts, and was essentially free from hyaluronic acid.
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Characterization of the link protein from bovine epiphyseal plate
TL;DR: Immunodiffusion studies of epiphyseal plate and articular links showed that the two tissues have immunologically indistinguishable link fractions.
Journal Article
Heterogeneity in Growth Properties of the Rat Swarm Chondrosarcoma
TL;DR: Of the Swarm rat chondrosarcoma, originating from a single neoplasm in a Sprague-Dawley rat more than thirty-five years ago, two populations were identified with different growth properties, and no obvious clues to the variation in growth rate were noted.
References
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Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis
Klaus Weber,Mary Osborn +1 more
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
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Automatic recording apparatus for use in the chromatography of amino acids.
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A modified uronic acid carbazole reaction
T. Bitter,Helen Muir +1 more
TL;DR: It has been found possible to distinguish betweenHeparin, heparin derivatives, and other polyuronides of connective tissue by comparing the effect of chlorides on the color yield in both procedures by modifying Dische's carbazole reaction for uronic acid in the presence of borate.
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Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates
TL;DR: Micromethods have been developed and 35S-labeled chondroitin sulfates A, B, and C in a given mixture have been precisely and rapidly determined by measuring radioactivity alone.