Isolation and characterization of proteoglycans from the swarm rat chondrosarcoma.
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Proteoglycan monomer (D1) and aggregate (A1) preparations were isolated from 4 M guanidinium chloride extracts of the Swarm rat chondrosarcoma and contained only small proteoglycan fragments, indicating that extensive enzymatic degradation had occurred.About:
This article is published in Journal of Biological Chemistry.The article was published on 1975-08-10 and is currently open access. It has received 543 citations till now. The article focuses on the topics: Proteoglycan & Benzamidine.read more
Citations
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Journal ArticleDOI
A murine tumor producing a matrix of basement membrane
TL;DR: A murine tumor previously classified as a poorly differentiated chondrosarcoma is studied, and it is shown at the ultrastructural level that the tumor matrix is a homogeneous, nonfibrillar material, resembling basement membrane.
Journal ArticleDOI
Culture and growth characteristics of chondrocytes encapsulated in alginate beads.
TL;DR: Preliminary studies with collagen and chitosan matrixes encapsulated in "hollow" beads suggest that cell growth and morphology are profoundly influenced by the composition of the cellular environment.
Journal ArticleDOI
Isolation and characterization of two sialoproteins present only in bone calcified matrix.
A Franzén,D Heinegård +1 more
TL;DR: Antigenicity of sialoprotein II was not affected by reduction and alkylation, was only partially lost upon trypsin digestion and was completely lost upon fragmentation of the core protein by alkaline-borohydride treatment, indicating that all antigenic sites were located in the protein portion.
Book ChapterDOI
Structure and Metabolism of Connective Tissue Proteoglycans
TL;DR: The segregation of the proteoglycans into a separate category is based on a few specific characteristics, including the fact that the d-glucuronic-acid-containing repeating disaccharide of chondroitin, N-acetylchondrosine, has recently been identified as a component of thyroglobulin.
Journal ArticleDOI
Identification of a monoclonal antibody that specifically recognizes corneal and skeletal keratan sulfate. Monoclonal antibodies to cartilage proteoglycan
TL;DR: The 1/20/5-D-4 monoclonal antibody appears to recognize a common determinant in their polysaccharide moieties, consistent with several biochemical analyses showing the absence of keratan sulfate in proteoglycan synthesised by this tissue.
References
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Journal ArticleDOI
A Comparison of Proteinpolysaccharides of Bovine Nasal Cartilage Isolated and Fractionated by Different Methods
TL;DR: Crude proteinpolysaccharide extractable by three fundamentally different methods, called mild, disruptive, and dissociative, has been separated into the same series of three significantly distinct fractions by a method capable of operating with gram quantities involving differential ultracentrifugation in neutral salt solutions at high concentrations.
Journal ArticleDOI
Proteoglycans from Bovine Proximal Humeral Articular Cartilage
TL;DR: Two states of macromolecular organization of proteoglycans appear to exist in articular cartilage, and the proteoglycan aggregate in either PG(MgCl2) or PG(GuHCl) is completely and reversibly dissociable in either 3 m MgCl 2 or 3 m GuHCl.
Journal ArticleDOI
Purification of Cathepsin D from Cartilage and Uterus and Its Action on the Protein-Polysaccharide Complex of Cartilage
TL;DR: It was finally shown that cathepsin D is completely inactive at pH 7.2 and that protein-polysaccharide complex digestion at this pH is due to the action of a contaminant neutral protease.
Journal ArticleDOI
The structure and composition of cartilage keratan sulphate
John J. Hopwood,H. Clem Robinson +1 more
TL;DR: Consideration of molecular parameters and compositions leads to a proposed structure for keratan sulphate-protein as found in skeletal proteoglycans, which is presented for two different types of linkage between skeletal keratan sulfate and protein.
Journal ArticleDOI
Connective Tissue Proteinpolysaccharides FRACTIONATION OF THE PROTEINPOLYSACCHARIDES FROM BOVINE NASAL CARTILAGE
Maureen D. Franek,J.R. Dunstone +1 more
TL;DR: A fractionation based on observed ultracentrifugal heterogeneities of proteinpolysaccharide preparations from bovine nasal cartilage has been devised and three glycosaminoglucuronoglycan-containing fractions and a glycoprotein fraction are isolated.